Fingerprint target regions were drawn from the literature and chosen based on their known and distinct connectivity, either strong or weak, with the seed region. Activity coupling in the control state is indicated in blue, following SMA TUS in red, and FPC TUS in yellow. (a) SMA connectional fingerprint. In the control state (blue) SMA is strongly coupled to areas in the dorsomedial sensorimotor system (M1, SPL, MCC). Coupling with each of these areas is enhanced after SMA TUS (red). In contrast, coupling with regions that SMA is weakly connected with in the control state are even further reduced after SMA TUS (red). However, SMA’s fingerprint is relatively unaffected after FPC TUS (yellow), but some effects are visible for regions that are strongly coupled with FPC (compare to panel b). (b) FPC connectional fingerprint is sharpened following FPC TUS, but not following SMA TUS. The latter only affected coupling with SMA itself and regions strongly connected to it (MCC). (c) TUS induced more homogenous activity within the stimulated area (SMA in red on the left, FPC in yellow on the right). (d–f) Temporal evolution of the effect of SMA TUS on the SMA connectional fingerprint. The left panel depicts effects observed in the first fMRI run, followed by the second run in the middle, and the third and last run on the right. For each run, the time after TUS refers to the duration between the end of TUS and the midpoint of the run, averaged across the three animals. (g–h) TUS-induced effects on the connectional fingerprints of SMA and FPC could not be explained by auditory activity. (i) There were no changes in the coupling between the stimulation sites and auditory cortex. In all plots, thick lines and histograms indicate mean activity coupling; lighter coloured error bands and error bars depict standard-error of the mean; the asterisk denotes the interaction between TUS and connectivity seed (p<0.001).