Genetic reagent (Drosophila melanogaster) | ova[1] | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | ova[4] | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | c587-GAL4 | (Song et al., 2004) (DOI: 10.1242/dev.01026) | RRID:BDSC_67747 | |
Genetic reagent (Drosophila melanogaster) | Dad-lacZ | (Tsuneizumi et al., 1997) (DOI: 10.1038/39362) | RRID:DGGR_118114 | |
Genetic reagent (Drosophila melanogaster) | bam-GFP | (Chen and McKearin, 2003) | RRID:DGGR_118177 | |
Genetic reagent (Drosophila melanogaster) | piwi[2] | (Lin and Spradling, 1997) | RRID:BDSC_43319 | |
Genetic reagent (Drosophila melanogaster) | piwi[3] | (Lin and Spradling, 1997) | RRID:BDSC_12225 | |
Genetic reagent (Drosophila melanogaster) | GFP-piwi | Katalin Toth (California Institute of Tchnology) | | |
Genetic reagent (Drosophila melanogaster) | 118E-10 | Lori Wallrath (University of Iowa) | | |
Genetic reagent (Drosophila melanogaster) | 118E-15 | Lori Wallrath (University of Iowa) | | |
Genetic reagent (Drosophila melanogaster) | 6 M-193 | Lori Wallrath (University of Iowa) | | |
Genetic reagent (Drosophila melanogaster) | 39C.72 | Lori Wallrath (University of Iowa) | | |
Genetic reagent (Drosophila melanogaster) | dLsd1-GFP | Yu Yang (Institute of Biophysics, Chinese Academy of Science) | | |
Genetic reagent (Drosophila melanogaster) | EGFP-RNAi | Bloomington Drosophila Stock Center | (#41553) | |
Genetic reagent (Drosophila melanogaster) | RFP-HP1a | Bloomington Drosophila Stock Center | (#30562) | |
Genetic reagent (Drosophila melanogaster) | UAS-Dcr2; nos-GAL4 | Bloomington Drosophila Stock Center | (#25751) | |
Genetic reagent (Drosophila melanogaster) | tub-GAL4 | Bloomington Drosophila Stock Center | (#5138) | |
Genetic reagent (Drosophila melanogaster) | tub-GAL80ts | Bloomington Drosophila Stock Center | (#7016, #7018) | |
Genetic reagent (Drosophila melanogaster) | Df(2L)BSC144 | Bloomington Drosophila Stock Center | (#9504) | |
Genetic reagent (Drosophila melanogaster) | attP2 | Bloomington Drosophila Stock Center | (#25710) | |
Genetic reagent (Drosophila melanogaster) | In(1)wm4h | Kyoto Stock Center | (#101652) | |
Genetic reagent (Drosophila melanogaster) | Df(2L)ED737 | Kyoto Stock Center | (#150520) | |
Genetic reagent (Drosophila melanogaster) | ova-RNAi | Vienna Drosophila Research Center | (#102156) | |
Genetic reagent (Drosophila melanogaster) | piwi-RNAi | Vienna Drosophila Research Center | (#101658) | |
Genetic reagent (Drosophila melanogaster) | panx-RNAi | Vienna Drosophila Research Center | (#102702) | |
Genetic reagent (Drosophila melanogaster) | EGFP-5xBoxB | Vienna Drosophila Research Center | (#313408) | |
Genetic reagent (Drosophila melanogaster) | lacO-GFP-Piwi | Vienna Drosophila Research Center | (#313394) | |
Genetic reagent (Drosophila melanogaster) | lacI-HP1a; lacO- GFP-Piwi | Vienna Drosophila Research Center | (#313409) | |
Genetic reagent (Drosophila melanogaster) | nos-Cas9 | Jianquan Ni (Tsinghua University) | | |
Genetic reagent (Drosophila melanogaster) | pCasper4-ova-g | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | GFP-ova | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-ova | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-piwi | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-ova1-249 | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-ova250-486 | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-ova388-623 | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-ova1-388 | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-HP1a::dLsd1 | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-λN-ova | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | UASP-lacI-ova | This paper | | See Materials and methods |
Genetic reagent (Drosophila melanogaster) | lacO-terminator-GFP-Piwi | This paper | | See Materials and methods |
Recombinant DNA reagent | UASP-λN | Julius Brennecke (Institute of Molecular Biotechnology) | | |
Recombinant DNA reagent | UASP-lacI | | |
Recombinant DNA reagent | lacO-GFP-Piwi | | |
Recombinant DNA reagent | pGBKT7 | Clontech (Cat#630443) | | |
Recombinant DNA reagent | pGAD | Clontech (Cat#630442) | | |
Antibody | rabbit polyclonal anti-pMad | Ed Laufer (Columbia Universtity Medical Center) | RRID:AB_2617125 | IHC(1:1000) |
Antibody | rabbit polyclonal anti-β-galactosidase | MP Biologicals (Cat#0855976) | RRID:AB_2687418 | IHC(1:3000) |
Antibody | mouse monoclonal anti-α-Spectrin | Developmental Studies Hybridoma Bank | | IHC(1:50) |
Antibody | mouse monoclonal anti-Tubulin | Developmental Studies Hybridoma Bank | RRID:AB_1157911 | WB(1:2000) |
Antibody | rabbit polyclonal anti-mCherry | BioVision (cat#5993) | RRID:AB_1975001 | WB (1:2000) |
Antibody | rabbit polyclonal anti-GFP | Life (cat#A11122) | RRID:AB_221569 | IHC(1:1000) WB(1:10000) |
Antibody | polyclonal anti-rabbit IgG-HRP | ZSJQ-BIO (cat#ZB2301) | | WB(1:10000) |
Antibody | rabbit polyclonal anti-H3K4me2 | Abcam (cat#ab7766) | RRID:AB_732924 | |
Antibody | mouse monoclonal anti-RNA polymerase II | Abcam (cat#ab817) | RRID:AB_306327 | |
Antibody | mouse monoclonal anti-Flag | Sigma (cat#F1804) | RRID:AB_439685 | IHC(1:300) WB(1:6000) |
Chemical compound, drug | 4’,6’-diamidino-2- phenylindole | Sigma (cat#10236276001) | | |
Commercial assay or kit | anti-Flag resin | Sigma (cat#A2220) | RRID:AB_10063035 | |
Commercial assay or kit | GFP-Trap agarose | Chromoteck (cat#gta-10) | | |
Commercial assay or kit | RFP-Trap agarose | Chromoteck (cat#rta-10) | | |
Commercial assay or kit | Qiagen Plasmid Midi Kit | Qiagen (#12145) | | |
Commercial assay or kit | Immobilon Western Chemiluminescent HRP Substrate Kit | Millipore (cat#WBKLS0500) | | |
Commercial assay or kit | HiScript II Q RT SuperMix | Vazyme Biotech (cat#R223-01) | | |
Commercial assay or kit | ChamQ SYBR qPCR master Mix | Vazyme Biotech (cat#Q331) | | |
Commercial assay or kit | Oligo d(T)25 Magnetic beads | NEB (cat#S1419S) | | |
Commercial assay or kit | NEBNext Ultra IIDNA Library Prep Kits for Illumina | NEB (cat# E7645S) | | |
Commercial assay or kit | VAHTS Small RNA Library Prep Kit for Illumina | Vazyme Biotech (cat#NR801) | | |
Commercial assay or kit | VAHTS Universal DNA Library Prep Kit | Vazyme Biotech (cat#ND607) | | |
Commercial assay or kit | TruePrep Index Kit | Vazyme Biotech (cat#TD202) | | |
Software, algorithm | GraphPad Prism | GraphPad Prism (https://graphpad.com) | RRID:SCR_002798 | |
Sequenced-based reagent | RT-qPCR primers | This paper | | See Supplementary file 1, Table 2 |