(A, B) Mammary gland sections of late E13 female and male embryos were stained with TrkBECD antibody and counterstained with DAPI for visualization of the mammary gland structure. Arrows indicate …
In-situ hybridization of mammary gland sections of late E13 female embryos using specific digoxigenin (DIG)-labeled RNA probes against Semaphorins of Class 3, 4, 5 and 6. Mammary epithelium is …
In-situ hybridization of mammary gland sections of late E13 female embryos using specific digoxigenin (DIG)-labeled Sense RNA probes against Semaphorins 3D, 3F and 6A. Mammary epithelium is marked …
(A, C, E) DRG explants from early E13 WT or PlexinA4 KO embryos were grown for 48 hr, treated with 1 ng/µl soluble Sema6A-Fc (A), 0.02 ng/µl soluble Sema3D-Fc (C), 1 nM of Sema3F-AP (E), or control …
Percentage of collapsed growth cones in WT and PlexinA4 KO.
DRG explants from early E13 embryos or PlexinA4 KO embryos grown for 48 hr were incubated with 1 ng/µl soluble Sema6A-Fc for 90 min containing 1:1000 goat anti-human IgG (Fc specific), Alkaline …
(A) Mammary gland sections of WT and Sema6A KO female embryos at different embryonic stages were stained with Tuj1 (red) and DAPI (blue) for visualization of sensory neuron innervations. Scale bar: …
Percentage of mammary gland innervation density in WT and Sema6A KO female embryos.
(A) Mammary gland sections of WT and Sema6A KO male embryos at different embryonic stages were stained with Tuj1 (red) and DAPI (blue) for visualization of sensory neuron innervations. Scale bar: 50 …
Percentage of mammary gland innervation density in WT and Sema6A KO male embryos.
(A) Mammary gland sections from WT, PlexinA4 KO, BDNF Het, and PlexinA4 KO/BDNF Het female embryos at different embryonic stages were stained with Tuj1 (red) and DAPI (blue) to visualize sensory …
Percentage of mammary gland innervation density in WT, PlexinA4 KO, BDNF Het and PlexinA4 KO/BDNF Het female embryos.
Mammary gland sections of late E13 WT and PlexinA4 KO female embryos were stained with TrkBECD antibody (red), Tuj1 (green) and counterstained with DAPI for visualization of the mammary gland …
(A) Mammary gland sections of WT, PlexinA4 KO, BDNF Het, and PlexinA4 KO/BDNF Het male embryos at different embryonic stages were stained with Tuj1 (red) and DAPI (blue) to visualize sensory neuron …
Percentage of mammary gland innervation density in WT, PlexinA4 KO, BDNF Het and PlexinA4 KO/BDNF Het male embryos.
Mammary gland sections of late E13 WT and PlexinA4 KO male embryos were stained with TrkBECD antibody (red), Tuj1 (green) and counterstained with DAPI for visualization of the mammary gland …
Mammary gland sections of late E13 WT and PlexinA4 KO male embryos were stained with TrkBECD antibody, and counterstained with DAPI for visualization of the mammary gland structure. Arrows indicate …
(A) Explants of early E13 embryonic DRGs were cultured in media containing 50 ng/ml BDNF for 48 hr, then the media was replaced to 50 ng/ml BDNF (+BDNF), or media without BDNF (-BDNF) both with low …
Percentage of collapsed growth cones.
(A) Mammary gland sections of WT, PlexinA4 KO, TrkB.T1 KO, and PlexinA4 KO/TrkB.T1 KO male embryos at different embryonic stages were stained with Tuj1 (red) and DAPI (blue) to visualize sensory …
Percentage of mammary gland innervation density in WT, PlexinA4 KO, TrkB.T1 KO and PlexinA4 KO/TrkB.T1 KO male embryos.
(A) In females: a balance between BDNF and Semaphorins specifies normal innervation of the mammary gland. Decreased levels of BDNF cause hypoinnervation and decreased Semaphorins cause …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (M. Musculus) | Sema6a-/- | PMID: 11242070 | Dr. Marc Tessier-Lavigne (Stanford) | |
Genetic reagent (M. Musculus) | Plxna4-/- | PMID: 15721238 | Dr. Marc Tessier-Lavigne (Stanford) | |
Genetic reagent (M. Musculus) | Ntrk2.T1-/- | PMID: 16815329 | Dr. Lino Tessarollo (NIH) | |
Genetic reagent (M. Musculus) | Bdnf-/- | Jackson Laboratory | stock #: 002266 RRID: MGI:2175720 | PMID: 8139657 |
Cell line (Homo sapiens) | HEK293 | ATCC | ATCC CRL-1573 RRID:CVCL_0045 | |
Cell line (Cercopithecus aethiops) | COS1 | ATCC | ATCC CRL-1650 RRID: CVCL_0223 | |
Antibody | Mouse monoclonal anti-tubulin β-III | R and D | Cat. #: MAB1195 RRID:AB_357520 | IHC (1:500) |
Antibody | Rabbit polyclonal anti-tubulin β-III | Biolegend | Cat. # 802001 RRID:AB_2564645 | IHC-Fr (1:1000) |
Antibody | Polyclonal Goat anti-Mouse Trkb | R and D | Cat. # AF1494 RRID:AB_2155264 | IHC-Fr (1:100) |
Peptide, recombinant protein | Human Sema6A-FC | PMID: 20606624 | ||
Peptide, recombinant protein | Mouse Sema3D-FC | This paper | ||
Peptide, recombinant protein | Mouse AP-Sema3F | PMID: 9331348 | ||
Chemical compound, drug | Phalloidin-Rhodamine | Sigma | p1951 | 1:250 |
Chemical compound, drug | Ara-C | Sigma | C1768 | 30 uM |