(A) Most frequent biological models used in main experiments within a sample of 100 Brazilian life sciences articles. (B) Most frequent methods used for quantitative outcome detection in these experiments. ‘Cell count’, ‘enzyme activity’ and ‘blood tests’ include various experiments for which methodologies vary and/or are not described fully in articles. Nociception tests, although frequent, were not considered for replication due to animal welfare considerations. (C) Flowchart describing the first full-text screening round to identify articles in our candidate techniques, which led us to select our final set of five methods.