Specification of diverse cell types during early neurogenesis of the mouse cerebellum
Abstract
We applied single-cell RNA sequencing to profile genome-wide gene expression in about 9,400 individual cerebellar cells from the mouse embryo at embryonic day 13.5. Reiterative clustering identified the major cerebellar cell types and subpopulations of different lineages. Through pseudotemporal ordering to reconstruct developmental trajectories, we identified novel transcriptional programs controlling cell fate specification of populations arising from the ventricular zone and the rhombic lip, two distinct germinal zones of the embryonic cerebellum. Together, our data revealed cell-specific markers for studying the cerebellum, gene-expression cascades underlying cell fate specification, and a number of previously unknown subpopulations that may play an integral role in the formation and function of the cerebellum. Our findings will facilitate new discovery by providing insights into the molecular and cell type diversity in the developing cerebellum.
Data availability
Sequencing data have been deposited in GEO under accession codes GSE120372.
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Sinle-cell RNA sequecing of E13.5 mouse cerebellaNCBI Gene Expression Omnibus, GSE120372.
Article and author information
Author details
Funding
NIH Office of the Director (R01NS106844)
- James YH Li
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Constance L Cepko, Harvard Medical School, United States
Ethics
Animal experimentation: All procedures involving animals were approved by the Animal Care Committee at the University of Connecticut Health Center and were in compliance with national and state laws and policies. (protocol #101849-0621
Version history
- Received: September 27, 2018
- Accepted: February 7, 2019
- Accepted Manuscript published: February 8, 2019 (version 1)
- Version of Record published: February 20, 2019 (version 2)
Copyright
© 2019, Wizeman et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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