Neuronal reactivation during post-learning sleep consolidates long-term memory in Drosophila

  1. Ugur Dag
  2. Zhengchang Lei
  3. Jasmine Q Le
  4. Allan Wong
  5. Daniel Bushey
  6. Krystyna Keleman  Is a corresponding author
  1. Janelia Research Campus, Howard Hughes Medical Institute, United States

Abstract

Animals consolidate some, but not all, learning experiences into long-term memory. Across the animal kingdom, sleep has been found to have a beneficial effect on the consolidation of recently formed memories into long-term storage. However, the underlying mechanisms of sleep dependent memory consolidation are poorly understood. Here, we show that consolidation of courtship long-term memory in Drosophila is mediated by reactivation during sleep of dopaminergic neurons that were earlier involved in memory acquisition. We identify specific fan-shaped body neurons that induce sleep after the learning experience and activate dopaminergic neurons for memory consolidation. Thus, we provide a direct link between sleep, neuronal reactivation of dopaminergic neurons, and memory consolidation.

Data availability

Source data files have been provided for Figure 1-figure supplement 1 and 2, Figure 2, Figure 2-figure supplement 1 and 2 and Figure 5 and Figure 5-figure supplement

Article and author information

Author details

  1. Ugur Dag

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6937-5722
  2. Zhengchang Lei

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6475-5010
  3. Jasmine Q Le

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4159-8830
  4. Allan Wong

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8492-2162
  5. Daniel Bushey

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Krystyna Keleman

    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    For correspondence
    kelemank@janelia.hhmi.org
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2044-1981

Funding

Howard Hughes Medical Institute (N/A)

  • Krystyna Keleman

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Mani Ramaswami, Trinity College Dublin, Ireland

Publication history

  1. Received: October 14, 2018
  2. Accepted: February 22, 2019
  3. Accepted Manuscript published: February 25, 2019 (version 1)
  4. Version of Record published: March 21, 2019 (version 2)

Copyright

© 2019, Dag et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Ugur Dag
  2. Zhengchang Lei
  3. Jasmine Q Le
  4. Allan Wong
  5. Daniel Bushey
  6. Krystyna Keleman
(2019)
Neuronal reactivation during post-learning sleep consolidates long-term memory in Drosophila
eLife 8:e42786.
https://doi.org/10.7554/eLife.42786

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