(A) ΔAMPs flies survive clean injury like wild-type flies, while ΔPPO mutants deficient for melanization have reduced survival over time. (B) ΔAMPs flies melanize the cuticle similar to wild-type flies following pricking (χ2 = 2.14, p=0.34). Melanization categories (None, Weak, Normal) were as described in Dudzic et al (Dudzic et al., 2018). Sample sizes (n) are included in each bar. (C) Melanization in iso w1118, ΔAMPs, and ΔPPO flies of the cuticle in adults (i, yellow arrowheads), larvae (ii, melanized wounds), and larval hemolymph (iii). (D) To investigate clotting ability, we used the hanging drop assay (Scherfer et al., 2004) with ΔAMPs larval hemolymph and visualized clot fibers with PNA staining (green). Both iso w1118 and ΔAMPs hemolymph produced visible clot fibres measured after 20 min. Hemocyte populations are normal in ΔAMPs flies, including crystal cell distribution (E) and number (F).