The width of each violin is scaled to the number of genes that are differentially expressed at each given time point (BH-adjusted p-value <0.01). Differentially expressed genes were defined as those …
Tissue data sets are presented as three coloured segments along the perimeter for the injected muscle (purple), the draining lymph nodes (green), and the blood compartment (red). An ordered list of …
Enrichment scores were calculated for blood transcriptional modules defined by Li et al. (2014) using R package tmod (version 0.34) (US National Library of Medicine, 2004). The modules (in rows) are …
Enrichment scores were calculated for blood transcriptional modules defined by Li et al. (2014) using R package tmod (version 0.34) (US National Library of Medicine, 2004). The modules (in rows) are …
Enrichment scores were calculated for blood transcriptional modules defined by Li et al. (2014) using R package tmod (version 0.34) (US National Library of Medicine, 2004). The modules (in rows) are …
Reference modules are the blood transcriptional modules defined by Li et al. (2014). These modules are shown in rows and are annotated within a higher functional group. Only WGCNA modules that …
Modules extracted by co-expression analyses in the injected muscle (A), draining lymph nodes (B), and blood (C) datasets are in rows labelled with a colour for each treatment and an identificaton …
The three tissues analysed are organised as in Figure 2 and are indicated on the outer part of the circular map. Each outer tile has a different colour to denote which injection they received: Fluad …
Serum chemokines and cytokines were measured using a Luminex bead 9-plex assay, and serum amyloid A3 was measured by quantitative ELISA. (A) Each panel shows the longitudinal expression of an individ…
Differential expression of transcripts and proteins induced by the LPS immunisation are shown. Each panel shows the fold changes (transcripts – green line; proteins – orange line) of individual …
The correlations between fold changes in transcript and protein levels are presented in this heatmap. All measured chemokine and cytokines are in rows (left axis legend). Columns represent different …
The vaccines, TLR agonists, adjuvant and saline used in the present study are detailed. Vaccines were administered by injection of 1/10th the human dose in a 50 µL volume. TLR agonists, IFA and …
Vaccine/TLR | Components | Abbreviation | Manufacturer |
---|---|---|---|
Pentavac SD | Diphtheria, tetanus, pertussis (whole cell), hepatitis B (rDNA) and haemophilus type b conjugate vaccine | Pentavac | Serum Institute India |
Agrippal | Trivalent flu subunits – H3N2, H1N1 and influenza B | Tri-Flu | Seqirus |
Fluad | Trivalent flu subunits – H3N2, H1N1 and influenza B + MF59 | Tri-Flu + MF59 | Seqirus |
Engerix B | Recombinant hepatitis B sAg absorbed on alum | GlaxoSmithKline | |
IFA | Montanide ISA 51 VG | Seppic | |
LPS | LPS-EB Ultrapure | Invivogen | |
Poly I:C | Polyinosinic:polycytidylic acid | Sigma |
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background) M. musculus, Female) | CB6F1/Crl; Strain Code 176 | Charles River | ||
Commercial assay or kit | RNAprotect animal blood tubes | Qiagen | Cat number: 76544 | |
Commercial assay or kit | miRNeasy mini kit | Qiagen | Cat number: 217004 | |
Commercial assay or kit | QIAzol lysis reagent | Qiagen | Cat number: 79306 | |
Commercial assay or kit | 9-plex Luminex | Bio-Techne | Cat number: LXSAMS-09 | |
Chemical compound, drug | Pentavac SD | Serum Institute India | ||
Chemical compound, drug | Agrippal | Seqirus | ||
Chemical compound, drug | Fluad | Seqirus | ||
Chemical compound, drug | Engerix B | GlaxoSmithKline | ||
Chemical compound, drug | IFA - Montanide ISA 51 VG | Seppic | NSC#737063 | |
Chemical compound, drug | LPS-EB Ultrapure | Invivogen | Cat number: tlrl-3pelps | |
Chemical compound, drug | Poly I:C | Sigma | Cat number: P1530 |