Cell-type specific innervation of cortical pyramidal cells at their apical dendrites
- Max Planck Institute for Brain Research, Germany
Abstract
We investigated the synaptic innervation of apical dendrites of cortical pyramidal cells in a region between layers (L) 1 and 2 using 3-D electron microscopy applied to four cortical regions in mouse. We found the relative inhibitory input at the apical dendrite's main bifurcation to be more than 2-fold larger for L2 than L3 and L5 thick-tufted pyramidal cells. Towards the distal tuft dendrites in upper L1, the relative inhibitory input was at least about 2-fold larger for L5 pyramidal cells than for all others. Only L3 pyramidal cells showed homogeneous inhibitory input fraction. The inhibitory-to-excitatory synaptic ratio is thus specific for the types of pyramidal cells. Inhibitory axons preferentially innervated either L2 or L3/5 apical dendrites, but not both. These findings describe connectomic principles for the control of pyramidal cells at their apical dendrites and support differential computational properties of L2,L3 and subtypes of L5 pyramidal cells in cortex.
Data availability
All 6 datasets are available for browsing at webknossos.org using the following links.S1: https://wklink.org/8732V2: https://wklink.org/9812PPC: https://wklink.org/1262ACC: https://wklink.org/6712LPtA: https://wklink.org/8912PPC2: https://wklink.org/6347All software used for analysis is available at (https://gitlab.mpcdf.mpg.de/connectomics/apicaltuftpaper) under the MIT license.
Article and author information
Author details
Moritz Helmstaedter
Funding
Max-Planck-Gesellschaft (Open-access funding)
- Ali Karimi
- Jan Odenthal
- Florian Drawitsch
- Kevin M Boergens
- Moritz Helmstaedter
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All experimental procedures were performed according to the law of animal experimentation issued by the German Federal Government under the supervision of local ethics committees and according to the guidelines of the Max Planck Society. The experimental procedures were approved by Regierungspräsidium Darmstadt, under protocol ID V54 - 19c20/15 F126/1015 (LPtA, PPC2) or V54 - 19 c 20/15 - F126/1002 (V2, PPC, ACC). The S1 sample was prepared following experimental procedures approved by Regierung von Oberbayern, 55.2-1-54-2532.3-103-12.
Copyright
© 2020, Karimi et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Cell-type specific innervation of cortical pyramidal cells at their apical dendrites
eLife 9:e46876.
https://doi.org/10.7554/eLife.46876
