1. Evolutionary Biology
  2. Genetics and Genomics
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Philosophy of Biology: The meanings of 'function' in biology and the problematic case of de novo gene emergence

  1. Diane Marie Keeling
  2. Patricia Garza
  3. Charisse Michelle Nartey  Is a corresponding author
  4. Anne-Ruxandra Carvunis  Is a corresponding author
  1. University of San Diego, United States
  2. Colegio de Saberes, Mexico
  3. University of Texas at Dallas, United States
  4. University of Pittsburgh, United States
  5. University of Pittsburgh School of Medicine, United States
Feature Article
Cite this article as: eLife 2019;8:e47014 doi: 10.7554/eLife.47014
1 figure, 3 tables and 1 additional file

Figures

Interpreting the word function in scientific abstracts related to de novo gene birth.

We analyzed a sample of 20 abstracts containing 42 instances where the word function or one of its derivatives was used to describe DNA, RNA or protein objects. First, each of us read the abstracts independently and assigned one or several of the meanings of function as defined in the Pittsburgh model to each of these instances. The distribution of the number of distinct meanings that we assigned to the 42 instances is shown in panel (A). For only 5 instances did all of us independently assign the same unique meaning, suggesting that function is most often interpreted in multiple ways by independent readers. Next, we discussed each instance to see if we could reach consensus assignments based on the textual evidence. Consensus was built through conversations and agreement between the readers, rather than majority opinion. The distribution of the number of unique meanings assigned after consensus agreement to each of the 42 instances is shown in panel (B). Most (26/42) instances are now assigned to a single meaning. When more than one meaning remains, the readers agreed that the textual evidence supported multiple meanings except for one instance where consensus could not be reached and three meanings were assigned to reflect all the differing interpretations of our team members. In panel C, we show the number of times each of the five meanings of function defined in the Pittsburgh model is assigned to an instance of function.

https://doi.org/10.7554/eLife.47014.003
Figure 1—source data 1

Independent and consensus assignments.

This table lists the results of our textual analyses for each instance of function.

https://doi.org/10.7554/eLife.47014.004

Tables

Table 1
The Pittsburgh model of function.

The hierarchical order of the meanings did not directly derive from our textual analysis, but was inspired from a reductionist interpretation of the flow of genetic information over time and space. It also reflects a possible ordering of the series of properties that must be acquired by a locus to undergo de novo gene birth.

https://doi.org/10.7554/eLife.47014.002
MeaningsDefinitions
Evolutionary ImplicationsThe object's influence on population dynamics over successive generations, as enabled by its physiological implications and their interplay with environmental pressures
Physiological ImplicationsThe object's involvement in biological processes as enabled by a set of its capacities, interactions and expression patterns, independent of cross-generational considerations
InteractionsPhysical contacts, direct or indirect, between the object under investigation and the other components of a system, including contacts that mediate chemical transformations
CapacitiesIntrinsic physical properties of the object under investigation; the necessity of the object's behavior given an environment (eg., structural constraints)
ExpressionThe presence or amount of the object under investigation (RNA or protein object), or the presence or amount of its transcription or translation products (DNA object)
VagueSufficient evidence was not found to infer one or more meanings of function within this model, nor to derive a new meaning
Table 2
References for 20 abstracts analyzed in our study.

Countries (based on affiliations of all authors) and model organisms are included to display the diversity of the abstracts.

https://doi.org/10.7554/eLife.47014.005
PapersCountriesModel Organisms
Keese, P. K., and Gibbs, A. (1992). Origins of genes: ‘big bang’ or continuous creation? PNAS 89:9489–9493.AustraliaCellular life, Viruses
Kastenmayer, J. P., Ni, L., Chu, A., Kitchen, L. E., Au, W. C., Yang, H.,. .. and Basrai, M. A. (2006). Functional
genomics of genes with small open reading frames (sORFs) in S. cerevisiaeGenome Research 16:365–373.
USAS. cerevisiae
Levine, M. T., Jones, C. D., Kern, A. D., Lindfors, H. A., and Begun, D. J. (2006). Novel genes derived from noncoding
DNA in Drosophila melanogaster are frequently X-linked and exhibit testis-biased expression. PNAS 103:9935–9939.
USAD. melanogaster
Stepanov, V. G., and Fox, G. E. (2007). Stress-driven in vivo selection of a functional mini-gene from a randomized
DNA library expressing combinatorial peptides in Escherichia coliMolecular Biology and Evolution 24:1480–1491.
USAE. coli
Cai, J., Zhao, R., Jiang, H., and Wang, W. (2008). De novo origination of a new protein-coding gene in Saccharomyces cerevisiae. Genetics 179:487–496.ChinaS. cerevisiae
Zhou, Q., Zhang, G., Zhang, Y., Xu, S., Zhao, R., Zhan, Z.,. .. and Wang, W. (2008). On the origin of new genes in Drosophila. Genome Research 18:1446–1455.ChinaDrosophila
Xiao, W., Liu, H., Li, Y., Li, X., Xu, C., Long, M., and Wang, S. (2009). A rice gene of de novo origin negatively
regulates pathogen-induced defense response. PLoS One 4:e4603.
China, USArice
Carvunis, A. R., Rolland, T., Wapinski, I., Calderwood, M. A., Yildirim, M. A., Simonis, N.,. ..and Vidal M. (2012).
Proto-genes and de novo gene birth. Nature 487:370–374.
Belgium, France, USAS. cerevisiae
Ding, Y., Zhou, Q., and Wang, W. (2012). Origins of new genes and evolution of their novel functions.
Annual Review of Ecology, Evolution, and Systematics 43:345–363.
China, USA
Tautz, D., Neme, R., and Domazet-Lošo, T. (2013). Evolutionary Origin of Orphan Genes. In: Encyclopedia of Life Sciences. John Wiley & Sons. DOI: https://doi.org/10.1002/9780470015902.a0024601Croatia, Germany
Reinhardt, J. A., Wanjiru, B. M., Brant, A. T., Saelao, P., Begun, D. J., and Jones, C. D. (2013). De novo ORFs in Drosophila are important to organismal fitness and evolved rapidly from previously non-coding
sequences. PLoS Genetics 9:e1003860.
USAD. melanogaster
Wissler, L., Gadau, J., Simola, D. F., Helmkampf, M., and Bornberg-Bauer, E. (2013). Mechanisms and dynamics
of orphan gene emergence in insect genomes. Genome Biology and Evolution 5:439–455.
Germany, USAInsects
Brylinski, M. (2013). Exploring the ‘dark matter’ of a mammalian proteome by protein structure and function modeling. Proteome Science 11:47.USAM. musculus
Li, D., Yan, Z., Lu, L., Jiang, H., and Wang, W. (2014). Pleiotropy of the de novo-originated gene MDF1.
Scientific Reports 4:7280.
ChinaS. cerevisiae
Wirthlin, M., Lovell, P. V., Jarvis, E. D., and Mello, C. V. (2014). Comparative genomics reveals molecular features
unique to the songbird lineage. BMC Genomics 15:1082. 
USASongbirds
Suenaga, Y., Islam, S. R., Alagu, J., Kaneko, Y., Kato, M., Tanaka, Y.,. .. and Nakagawara, A.(2014).
NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3β resulting in the stabilization
of MYCN in human neuroblastomas. PLoS Genetics 10:e1003996.
JapanHuman
Arendsee, Z. W., Li, L., and Wurtele, E. S. (2014). Coming of age: Orphan genes in plants.
Trends in Plant Science 19:698–708.
USAA. thaliana
Ruiz-Orera, J., Hernandez-Rodriguez, J., Chiva, C., Sabidó, E., Kondova, I., Bontrop, R.,. .. and Albà, M. M. (2015).
Origins of de novo genes in human and chimpanzee. PLoS Genetics 11:e1005721.
Spain, The NetherlandsHuman, Chimpanzee
Couso, J. P., and Patraquim, P. (2017). Classification and function of small open reading frames.Nature Reviews Molecular Cell Biology 18:575–589.Spain, UKD. melanogaster
Luis Villanueva-Cañas, J., Ruiz-Orera, J., Agea, M. I., Gallo, M., Andreu, D., and Albà, M. M. (2017). New genes and functional innovation in mammals. Genome Biology and Evolution 9:1886–1900.SpainMammals
Table 3
Examples of each meaning of function as assigned to instances of usage.

Underlined portions of sentences serve as the contextual evidence used to assign the ‘code’, or meaning, to the bolded instances analyzed.

https://doi.org/10.7554/eLife.47014.006
ReferenceInstance of function usageConsensus meanings
Wirthin et al., 2014‘Here we performed a comparative analysis of 48 avian genomes to identify genomic features that are unique to songbirds, as well as an initial assessment of function by investigating their tissue distribution and predicted protein domain structure.’Expression, Capacities
Brylinski, 2013A subsequent structure-based function annotation of small protein models exposes 178,745 putative protein-protein interactions with the remaining gene products in the mouse proteome, 1,100 potential binding sites for small organic molecules and 987 metal-binding signatures.Interaction
Li et al., 2014‘Therefore, MDF1 functions in two important molecular pathways, mating and fermentation, and mediates the crosstalk between reproduction and vegetative growth.’Physiological Implications
Ruiz-Orera et al.,
2015
‘In general, these transcripts show little evidence of purifying selection, suggesting that many of them are not functionalEvolutionary Implications

Data availability

All data analyzed in this manuscript consists of published research abstracts that are freely available online. Source data file has been provided for Figure 1.

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