(A) Embryonic width plotted vs. embryonic length in three genotypes. (B) Embryo aspect ratio (length/width) for OreR and fat2RNAi embryos measured from live and fixed embryos (n > 200 in all conditions). Error bars, standard deviation. (C) Comparison of mean embryo length and width for OreR (red) and fat2RNAi (blue) embryos collected from live and fixed embryos. Error bars, standard deviation. n > 200 in all conditions. (D) Rate of embryonic hatching, puparation and adult emergence in wild type, fat2RNAi individuals longer or shorter than 400 µm, 6xbcd and 10xbcd embryos. (E) Max projection of Dapi staining in wild type and fat2RNAi embryos. Scale bar, 100 µm. (F) Representative images of in situ against bcd in wild type and fat2RNAi embryos at n.c. 4, 8 and 12. (G) Effect of protein folding time on gradient profiles in SDD model. Predicted functional Bcd concentration in fat2RNAi (blue) and ctrl (red) embryos with fast folding (assuming immediate folding, dashed line) or slow folding (~50 min, solid line) plotted against absolute distance from anterior (top panel) or relative AP positions (mid panel). Same normalization is used as in Figure 1K. The concentration difference in the fast folding scenario at each relative AP position is plotted in the lower panel. Scale bars, 50 µm.