Structured inhibitory activity dynamics in new virtual environments

Abstract
Data availability
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Abstract

Inhibition plays a powerful role in regulating network excitation and plasticity; however, the activity of defined interneuron types during spatial exploration remain poorly understood. Using two-photon calcium imaging, we recorded hippocampal CA1 somatostatin- and parvalbumin-expressing interneurons as mice performed a goal-directed spatial navigation task in new visual virtual reality (VR) contexts. Activity in both interneuron classes was strongly suppressed but recovered as animals learned to adapt the previously learned task to the new spatial context. Surprisingly, although there was a range of activity suppression across the population, individual somatostatin-expressing interneurons showed consistent levels of activity modulation across exposure to multiple novel environments, suggesting context-independent, stable network roles during spatial exploration. This work reveals population-level temporally dynamic interneuron activity in new environments, within which each interneuron shows stable and consistent activity modulation.

Data availability

Source data are available at Dryad digital repository under the DOI 10.5061/dryad.f83kt85. Code to analyse the data has been deposited to GitHub at https://github.com/Han-Lab-WUSM/MA-scripts (commit 54efc13).

The following data sets were generated

1. Arriaga M
2. Han E
(2019) Data from: Structured Inhibitory Activity Dynamics During Learning
Dryad Digital Repository, 10.5061/dryad.f83kt85.

https://dx.doi.org/10.5061/dryad.f83kt85

Article and author information

Author details

Moises Arriaga

Department of Neuroscience, Washington University School of Medicine, St Louis, United States

Competing interests
The authors declare that no competing interests exist.
Edward B Han

Department of Neuroscience, Washington University School of Medicine, St Louis, United States

For correspondence
ehan23@wustl.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-1009-2186

Funding

McDonnell Center for Systems Neuroscience

Edward B Han

McDonnell Center for Cellular and Molecular Neurobiology

Edward B Han

Cognitive, Computational, Systems Neuroscience Pathway at Washington University in St. Louis (Graduate Student Fellowship)

Moises Arriaga

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols of Washington University (Animal Welfare Assurance # A-3381-01). The protocol was approved by the Washington University School of Medicine IACUC (#20170230). All surgery was performed under isofluorane anesthesia, and every effort was made to minimize suffering.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.