(A) Representative images of AWA, AWC and AWB cilia in wild-type and tub-1(nr2004) mutants. Cartoons of cilia morphologies are shown at top. Numbers at top right (in AWA and AWC image panels) …
Data for Figure 1B and E, and Figure 1—figure supplement 1.
Representative images (top) and quantification (bottom) of AWB cilia lengths in animals of the indicated genotypes. Human (Hs - Homo sapiens) GFP::TULP1 and GFP::TULP3-encoding sequences were …
(A–G) Representative images of localization patterns of the indicated fusion proteins in wild-type (WT) and tub-1(nr2004) mutants in AWB (A–E), ASK (F) and ASH (G). The AWB, ASK, and ASH neurons …
Representative images of str-1p::mCherry and str-1p::TAX-4::GFP in AWB neurons of wild-type and odr-3(Q206L)XS (Roayaie et al., 1998) animals. The AWB neuron is marked via expression of str-1p::mCher…
(A) Representative images of tagRFP::TUB-1 localization in AWB indicating cytoplasmic localization in the soma (open orange arrow) and enrichment at the dendritic ends (yellow arrowheads; left and …
Data for Figure 3D,E,F, and Figure 3—figure supplement 1A.
(A) Representative images (left) and quantification of AWB cilia lengths (right) in animals of the indicated genotypes. Alleles used were tub-1(nr2004) and daf-10(e1387). *** indicates different …
(A) (Top) Representative images of AWB cilia morphologies in animals of the indicated genotypes. Alleles used were tub-1(nr2004) and odr-1(n1936). The AWB neurons were visualized via expression of st…
Data for Figure 4A,B,D,E, and Figure 4—figure supplement 1.
Representative images (top) and quantification of maximum AWB cilia widths in animals of the indicated genotypes. *** and &&& indicate different from wild-type and odr-1, respectively, at p<0.001 …
(A, C, D) Representative images of GFP::PLCδ1-PH (A), tagRFP::TUB-1 (C) and GFP::PPK-1 (D) localization in AWB cilia of animals of the indicated genotypes. Alleles used were odr-1(n1936) and inpp-1(g…
Data for Figures 5B and E, and Figure 5—figure supplement 1.
(A) Representative images of INPP-1A::GFP localization in AWB cilia of animals of the indicated genotypes. Yellow and white arrowheads indicate the cilia base and cilia, respectively; arrow …
(A, D) Representative images of GFP::PPK-1 (A) and GFP::PLCδ1-PH (D) localization in animals of the indicated genotypes. Alleles used were tub-1(nr2004) and odr-1(n1936). The AWB neuron is marked …
Data for Figure 6B,C,E,F, Figure 6—figure supplement 1, and Figure 6—figure supplement 2B and C.
Representative images (left) and line scans (right) of GFP::PLCδ1-PH distribution in AWB cilia and dendrites in animals of the indicated genotypes. Yellow and white arrowheads indicate the cilia …
(A) Representative images of GFP::TUB-1 localization in the ASK neurons and enrichment at the dendritic ends (yellow arrowheads). ASK soma is indicated by an open orange arrowhead. GFP::TUB-1 is …
(A, B) (Left) Representative images of DPY-23::GFP (A) and GFP::PLCδ1-PH (B) localization in AWB cilia of animals of the indicated genotypes. AWB cilia were visualized via expression of str-1p::mCher…
Data for Figure 7A–D and Figure 7—figure supplement 1A–C.
(A–C) Representative images (left) and quantification of fluorescence intensities (right) of the indicated proteins at the PCMCs of AWB (A) and ASK (B and C). (Left images) Neurons are marked via …
Cartoons of proposed TUB-1 functions at the PCMC and within AWB cilia. Dotted yellow bars indicate singlet microtubules present in the distal segments of AWB cilia; the origins of these singlet …
List of strains used in this work.
List of plasmids used in this work.