(A, D) Representative images of GFP::PPK-1 (A) and GFP::PLCδ1-PH (D) localization in animals of the indicated genotypes. Alleles used were tub-1(nr2004) and odr-1(n1936). The AWB neuron is marked via expression of str-1p::GFP::PPK-1::SL2::mScarlet or GFP::PLCδ1-PH::SL2::mScarlet (A, D, respectively). Yellow and white arrowheads indicate the cilia base and cilia, respectively; arrow indicates the dendrite. Scale bars: 5 μm. (B, E) Line scans of GFP::PPK-1 (B) and GFP::PLCδ1-PH (E) intensities in AWB dendrites of animals of the indicated genotypes. Zero indicates the cilia base/dendritic tip. Fluorescence intensities were normalized to the maximum intensity for each individual animal across the measured region, and the percent of this maximum intensity was calculated at each location to create individual line scans. Error bars are the SEM; n ≥ 30 neurons each. (C, F) Relative fluorescence intensities of GFP::PPK-1 (C) and GFP::PLCδ1-PH (F) in AWB cilia vs dendrites in animals of the indicated genotypes. Open circles indicate data repeated from Figure 5. *, ** and *** indicate different from wild-type at p<0.05, p<0.01, and p<0.001; & and &&& indicate different from odr-1 at p<0.05 and p<0.001, (ANOVA and post-hoc Tukey’s test). Each dot represents measurements from a single AWB neuron. Horizontal line is mean; error bars are SEM.