(A) Relationship between cDNA yields, number of sorted cells, mapped reads, number of detected genes with more than one mapped read. Data for each biological replicate are shown as individual symbols. Original data are presented in Supplementary file 1. (B) Quantification of NOS immunoreactivity (a.u.; arbitrary units) in the γ1 or α2 compartment with or without NOS-RNAi. The whiskers represent the minimum and maximum. N = 12. Asterisk indicates significance from 0: *, p<0.05; n.s., not significant. Horizontal dotted line indicates the mean level of NOS immunoreactivity in the brain. (C) Outside of the MB, only a couple of additional DANs are labeled by the NOS FISH probes. Colabeling by FISH probes against TH (green) and first 4 exons of NOS1 transcripts (magenta) is shown. Diagram and projection of anterior or posterior brains are shown. Circles in diagram represent soma of DANs detected by TH probes; the different clusters of dopaminergic cell bodies (PAM, PAL, T1, PPM1, PPM2, PPM3, PPL1, PPL2c, PPL2ab and VUM) are indicated. Circles filled with magenta represent DANs overlapping with NOS (arrows). In addition to one cell in PPL1 (presumably PPL1-γ1pedc) and about a dozen of PAM cluster cells, we observed that one cell in the PPL2ab cluster and a small TH-positive cell outside, but near, the PPL2ab cluster (arrows) were labeled with NOS-FISH probes. (D) NOS-FISH signal was observed in soma of PPL1-γ1pedc but not in PPL1-α′2α2.