(A) Coupling response curves for Vehicle, VU, BU, and CLP290 treatment (n = 6 L20 slices/antagonist, see Figure 4—figure supplement 1 for cellular sample sizes). (B) Average phase maps depicting SCN spatiotemporal organization on Cycle 2 (C2) and Cycle 5 (C5) in vitro illustrating drug-induced effects on the magnitude and direction of network coupling. (C) Average period maps illustrating region-specific effects on cellular period. (D) Average period length (± SEM) of SCN shell and core neurons provided Vehicle, VU, BU, or CLP290 treatment while in the reorganized state induced by L20 (i.e., core-shell relationship = −11 hr ± 2 hr). See Figure 4—figure supplement 1 for full period response curves. Below X-axis: Schematic illustrating how network coupling is altered by region-specific modulation of period responses, with the net effect on the direction of network resynchronization indicated within each polar plot. * Differs from Vehicle control, post-hoc Least Square Means contrasts, p<0.01 (color-coded by antagonist in 4A), + Differs from within-group complementary region, LSM contrasts, p<0.05. See Supplementary file 2 for full statistical results. Other conventions as in Figure 3.