Dendritic NMDA receptors in parvalbumin neurons enable strong and stable neuronal assemblies

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Tables

Key resources table

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Mus musculus)	B6;129P2-Pvalbtm1(cre)Arbr/J	The Jackson Laboratory	008069
Strain, strain background (Mus musculus)	B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze	The Jackson Laboratory	007909
Other	AAV5-CaMKIIa-hChR2(H134R)-EYFP	UNC Vector Core
Chemical compound, drug	Picrotoxin	Sigma-Aldrich	P1675
Chemical compound, drug	CGP 55845	Abcam	Ab120337
Chemical compound, drug	D-AP5	Tocris	0105
Chemical compound, drug	TTX	Tocris	1078
Chemical compound, drug	ZD 7288	Tocris	1000
Chemical compound, drug	MNI-glutamate TFA	Femtonics	1951

Table 1

Parameter	Proximal	Distal	Units
Cm	0.9	0.9	µF cm-2
Raxial	170	170	Ω cm
Rm	5.55	55.5	kΩ
eleak	-65	-	mV
egk	-90	-	mV
egNa	55	-	mV
v Shift	-12	-12	mV
gk dend	300	300	pS µm-2
gNa dend	200	100	pS µm-2
gNa Soma	2000	-	pS µm-2

Table 2

Parameter	FS PV+	Pyramidal	Units
${\mathit{C}}_{\mathit{m}}$	90	115	pF
${\mathit{k}}_{\mathit{l}\mathit{o}\mathit{w}}$	1.7	0.1	nS/mV
${\mathit{k}}_{\mathit{h}\mathit{i}\mathit{g}\mathit{h}}$	14	3.3	nS/mV
${\mathit{v}}_{\mathit{r}}$	−60.6	−65.8	mV
${\mathit{v}}_{\mathit{p}\mathit{e}\mathit{a}\mathit{k}\mathit{}}$	2.5	22.6	mV
${\mathit{v}}_{\mathit{t}}$	−43.1	−57	mV
$\mathit{a}$	0.1	0.0012	ms−1
$\mathit{b}$	−0.1	3	nS
$\mathit{c}$	−67	−65.8	mV
$\mathit{d}$	0.1	10	pA
${\mathit{\tau }}_1^{\mathit{A}\mathit{M}\mathit{P}\mathit{A}}$	0.25	0.2	ms
${\mathit{\tau }}_2^{\mathit{A}\mathit{M}\mathit{P}\mathit{A}}$	0.77	1.7	ms
${\mathit{\tau }}_1^{\mathit{G}\mathit{A}\mathit{B}\mathit{A}}$	0.27	0.3	ms
${\mathit{\tau }}_2^{\mathit{G}\mathit{A}\mathit{B}\mathit{A}}$	1.7	3.5	ms
${\mathit{\tau }}_1^{\mathit{N}\mathit{M}\mathit{D}\mathit{A}}$	2	-	ms
${\mathit{\tau }}_2^{\mathit{N}\mathit{M}\mathit{D}\mathit{A}}$	60	-	ms
${\mathit{e}}^{\mathit{g}\mathit{l}\mathit{u}}$	0	0	mV
${\mathit{e}}^{\mathit{G}\mathit{A}\mathit{B}\mathit{A}}$	−70	−70	mV
${\mathit{}{\mathit{\sigma }}_{\mathit{D}}}^2$	0.015/(npyr2)	-	-
${\mathit{C}}_{\mathit{s}\mathit{y}\mathit{n}}$	9	-	pF
${\mathit{k}}^{\mathit{s}\mathit{y}\mathit{n}}$	3/npyr	-	-
${\mathit{g}}^{\mathit{l}\mathit{e}\mathit{a}\mathit{k}}$	5	-	nS
${\mathit{e}}^{\mathit{l}\mathit{e}\mathit{a}\mathit{k}}$	−60.6	-	mV
${\mathit{k}}^{\mathit{A}\mathit{M}\mathit{P}\mathit{A}}$	28	-	-
${\mathit{k}}^{\mathit{N}\mathit{M}\mathit{D}\mathit{A}}$	212	-	-
${\mathit{k}}^{\mathit{G}\mathit{A}\mathit{B}\mathit{A}}$	28	27	-
${\mathit{k}}^{\mathit{e}\mathit{x}\mathit{t}}$	5	1	-

1. Izhikevich Parameter values as in Ferguson et al. (2014) and Ferguson et al. (2013). Synaptic time constants: (Bartos et al., 2002; Geiger et al., 1997; Roth and van Rossum, 2009). Remaining parameters where adjusted to allow the network to generate a gamma rhythm.

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