Kv1.1 contributes to a rapid homeostatic plasticity of intrinsic excitability in CA1 pyramidal neurons in vivo
- Institute of Neurobiology of the Mediterranean Sea (INMED), Turing Center for Living Systems (CENTURI), Aix-Marseille University, INSERM, France
- Institut Universitaire de France, France
Figures
Figure 1 with 1 supplement
Theta-burst stimulation reduces intrinsic excitability.
(A) Left In vivo whole-cell recordings were made from CA1 pyramidal neurons. The TBS was applied through the patch pipette. Right An example of TBS. EPSP-like waveforms were applied in bursts of 10 …
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Figure 1—source data 1
Spike count in TBS and control conditions.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig1-data1-v1.xlsx
Figure 1—figure supplement 1
Change in excitability is not related to inital state of the cells.
(A) Correlation with the input resistance prior to TBS. (B) Correlation with membrane potential immediately after breaking in. (C) Correlation with initial AP threshold. (D) Correlation with …
Figure 2 with 2 supplements
Reduction in excitability is associated with decreased Rin and increased latency to first spike.
(A) Example traces showing a reduction in input resistance. Each trace is the mean of 10 traces. Rin was calculated on hyperpolarizing steps only and the voltage was taken as the minimum within the …
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Figure 2—source data 1
Input resistance and first spike latency in TBS and control conditions.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig2-data1-v1.xlsx
Figure 2—figure supplement 1
TBS did not change background synaptic activity.
(A) Examples of synaptic activity prior to testing excitability before and 20 minutes post-TBS. (B) Membrane potential variance prior to and 20 minutes post-TBS. (C) Scatter plot showing no …
Figure 2—figure supplement 2
Action potential threshold and medium AHP.
(A) Action potential threshold prior to and 20 minutes post-TBS. (B) Comparison of change in threshold between TBS and control cells. (C) AHP amplitude prior to and 20 minutes post-TBS. (D) …
Figure 3
Calcium chelation prevents the TBS-induced decrease in excitability.
(A-B) Example cell showing that the decrease in excitability is blocked with 0.5mM BAPTA inside the patch pipette. Scatter plots show the response to each of the maximum current injections during …
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Figure 3—source data 1
Spike count, input resistance and first spike latency in TBS condition with BAPTA.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig3-data1-v1.xlsx
Figure 4
NMDAR block does not prevent the TBS-induced decrease in excitability.
(A-B) Example cell showing that 1mM MK-801 inside the pipette does not prevent the decrease in excitability. Scatter plot shows the response to each of the maximum current injections during the …
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Figure 4—source data 1
Spike count, input resistance and first spike latency in TBS condition with MK801.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig4-data1-v1.xlsx
Figure 5
Changes in Ih do not account for decrease in excitability.
(A) Example traces showing the voltage sag caused by Ih pre and 20 min post-TBS. Each trace is the mean of 10 trials. (B) Sag ratio prior to and 20 minutes post-TBS (n = 16). (C) Sag ratio prior to …
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Figure 5—source data 1
Sag ratio in TBS condition without drugs and with BAPTA and MK801.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig5-data1-v1.xlsx
Figure 6
Block of Ih does not prevent reduction in excitability.
(A-B) Inclusion of ZD7288 in the patch pipette blocked Ih, abolishing the voltage sag. Each trace shown is the mean of 10 responses. (C-D) Example cell showing the effect of TBS in the presence of …
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Figure 6—source data 1
Spike count, input resistance and first spike latency in TBS condition with ZD7288.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig6-data1-v1.xlsx
Figure 7
Dendrotoxin-K reduces the effect of TBS on excitability.
(A-B) Example cell recorded after the application of DTX-K. Scatter plot shows the response to each of the maximum current injections during the testing protocol (grey circles). The red circles mark …
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Figure 7—source data 1
Spike count, input resistance and first spike latency in TBS condition with DTX-K.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig7-data1-v1.xlsx
Figure 8 with 1 supplement
Changes in excitability during exploration of a novel virtual reality track.
(A) Firing rate map of one cell. Lighter colors indicate higher rate. (B) In-field and out-of-field firing rate by lap for the example cell. (C) Mean firing rates outside the place field across the …
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Figure 8—source data 1
In-field, out-of-field firing rates and correlations across laps for place cells in new condition.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig8-data1-v1.xlsx
Figure 8—figure supplement 1
Changes in excitability during exploration of a familiar environment.
(A) Mean firing rates outside the place field across the session. (B) Distribution of the out-of-field firing rate correlations. (C) Mean in-field firing rates across the session. (D) Distribution …
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Figure 8—figure supplement 1—source data 1
In-field, out-of-field firing rates and correlations across laps for place cells in familiar condition.
- https://cdn.elifesciences.org/articles/49915/elife-49915-fig8-figsupp1-data1-v1.xlsx
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | ZD7288 | Tocris Bioscience | Cat. No. 1000 | |
| Chemical compound, drug | Dendrotoxin K | Sigma Aldrich | D4183 | |
| Chemical compound, drug | MK-801 | Tocris Bioscience | Cat. No. 0924 |
