Roles of C/EBP class bZip proteins in the growth and cell competition of Rp (‘Minute’) mutants in Drosophila

  1. Jorge Blanco
  2. Jacob C Cooper
  3. Nicholas E Baker  Is a corresponding author
  1. Albert Einstein College of Medicine, United States
  2. University of Utah, United States
8 figures and 2 additional files

Figures

Xrp1 and related bZip proteins in cell competition.

(A) Known dimers of Drosophila C/EBP –class bZip proteins and their potential functions. (B,C) Mitotic recombination in RpL36+/+ wing discs (grey) generates clones of RpL36+/+/+ cells (light grey) …

dATF4/crc requirement in wild type and Rp+/- wing discs.

(A) In the TIE-DYE method, independent recombination events generate parallel clones expressing GFP (green), Gal4 (detected through UAS-RFP expression in red), and b-Gal (blue). These parallel …

Cross-regulation of Xrp1 and IRBP18 expression.

(A,B) Xrp1 protein (labeled in B) is elevated in RpS18+/- cells (green in A) compared to RpS18+/+ cells (unlabelled in A). (C,D) Irbp18 protein (labeled in D) is elevated in RpS18+/- cells (green in …

Figure 4 with 1 supplement
Modifications of the endogenous Xrp1 locus and their consequences for cell competition.

(A) Sequential modifications of the Xrp1 locus in the 18925–18926 kb region of chromosome arm 3R by homologous recombination, Cre recombination and PhiC31 integration introduced modified and …

Figure 4—figure supplement 1
Xrp1attP flox is a loss-of-function allele.

(A) As clones of RpS18+/+ cells (unlabelled) expand to occupy most of a RpS18+/- wing imaginal disc (green, GFP labeling), apoptosis is elevated especially at the boundaries between the cells …

Ectopic Xrp1 requires IRBP18 to affect clone survival.

(A) Wing imaginal discs containing clones of control cells lacking b-galactosidase (magenta) and reciprocal clones expressing GFP (green). In panels B-D) Xrp1 is co-expressed with GFP. Xrp1 led to …

Figure 6 with 1 supplement
Xrp1 over-expression affects the eye in an irbp18-dependent manner.

Most panels show adult eyes where GMR-Gal4 has been used to express the indicated proteins and dsRNAs posterior to the morphogenetic furrow. Temperature was 25°C except where indicated otherwise. …

Figure 6—figure supplement 1
Cell cycle and genetic interactions in the GMR>Xrp1 genotype.

(A) GMR-Gal4 drives UAS-GFP expression (green) posterior to the morphogenetic furrow in the eye imaginal disc. An antibody specific for the Xrp1 long isoform detects co-expressed Xrp1 protein …

Figure 7 with 1 supplement
Ectopic expression of mutated Xrp1 proteins.

(A–L) Over-expression of Xrp1 proteins during wing development using nub-Gal4 at 18°C. A,C,E,G,I,K show wings from males misexpressing the indicated proteins. Results were similar from females …

Figure 7—figure supplement 1
Xrp11-546 expression at higher temperature.

(A) Scanning Electron Micrograph (SEM) of male GMR-Gal4/+ eye, raised at 25°C. Morphology is almost wild type (compare Figure 7M). (B) At 25°C, expression of the Xrp11-546 protein that lacks the …

Figure 8 with 1 supplement
Xrp1 gene conservation.

(A) PAML results for 12 Drosophila species showing the location of sites under strong positive selection in the Xrp1 protein. (B) The conservation (% amino-acid identity to Xrp1 from Drosophila …

Figure 8—figure supplement 1
Clustal O alignment of protein sequences from the Dipteran insects Drosophila melanogaster, Drosophila virilis, Musca domestica, Stomoxys calcitrans, Lucilia cuprina, Bactrocera dorsalis, and Glossina morsitans.

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