Cell type specific control of basolateral amygdala neuronal circuits via entorhinal cortex-driven feedforward inhibition
- University of Colorado Anschutz Medical Campus, United States
Abstract
The basolateral amygdala (BLA) plays a vital role in associating sensory stimuli with salient valence information. Excitatory principal neurons (PNs) undergo plastic changes to encode this association; however, local BLA inhibitory interneurons (INs) gate PN plasticity via feedforward inhibition (FFI). Despite literature implicating parvalbumin expressing (PV+) INs in FFI in cortex and hippocampus, prior anatomical experiments in BLA implicate somatostatin expressing (Sst+) INs. The lateral entorhinal cortex (LEC) projects to BLA where it drives FFI. In the present study, we explored the role of interneurons in this circuit. Using mice, we combined patch clamp electrophysiology, chemogenetics, unsupervised cluster analysis, and predictive modeling and found that a previously unreported subpopulation of fast-spiking Sst+ INs mediate LEC→BLA FFI.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
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Author details
Funding
National Institutes of Health (NS095311)
- Molly M Huntsman
National Science Foundation (DGE-1553798)
- E Mae Guthman
National Institutes of Health (DC000566)
- Diego Restrepo
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (#00039) of the University of Colorado Denver | Anschutz Medical Campus.
Copyright
© 2020, Guthman et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Cell type specific control of basolateral amygdala neuronal circuits via entorhinal cortex-driven feedforward inhibition
eLife 9:e50601.
https://doi.org/10.7554/eLife.50601
