Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila

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Abstract

An efficient vectorial intracellular transport machinery depends on a well-established apico-basal polarity and is a prerequisite for the function of secretory epithelia. Despite extensive knowledge on individual trafficking pathways, little is known about the mechanisms coordinating their temporal and spatial regulation. Here, we report that the polarity protein Crumbs is essential for apical plasma membrane phospholipid-homeostasis and efficient apical secretion. Through recruiting β_Heavy-Spectrin and MyosinV to the apical membrane, Crumbs maintains the Rab6-, Rab11- and Rab30-dependent trafficking and regulates the lipid phosphatases Pten and Ocrl. Crumbs knock-down results in increased apical levels of PI(4,5)P₂ and formation of a novel, Moesin- and PI(4,5)P₂-enriched apical membrane sac containing microvilli-like structures. Our results identify Crumbs as an essential hub required to maintain the organization of the apical membrane and the physiological activity of the larval salivary gland.

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We provide as "Source data" files all the data used for statistical analyses and generation of all graphs. These files are sorted according to the Figure and the corresponding supplemental figures, which correspond to Figure 1 and its supplements, Figure 2, Figure 3 and its supplements, Figure 5 and its supplements, and Figure 6

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Johanna Lattner

Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
No competing interests declared.

"This ORCID iD identifies the author of this article:" 0000-0003-3421-9134
Weihua Leng

Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
No competing interests declared.
Elisabeth Knust

Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Competing interests
Elisabeth Knust, Reviewing editor, eLife.

"This ORCID iD identifies the author of this article:" 0000-0002-2732-9135
Marko Brankatschk

The Biotechnological Center, Technische Universität Dresden, Dresden, Germany

For correspondence
marko.brankatschk@tu-dresden.de

Competing interests
No competing interests declared.

"This ORCID iD identifies the author of this article:" 0000-0001-5274-4552
David Flores-Benitez

Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

For correspondence
flores@mpi-cbg.de

Competing interests
No competing interests declared.

"This ORCID iD identifies the author of this article:" 0000-0001-8244-9335

Funding

Max-Planck-Gesellschaft

Johanna Lattner
Weihua Leng
Elisabeth Knust
David Flores-Benitez

Deutsche Forschungsgemeinschaft (BR5490/2)

Marko Brankatschk

Deutsche Forschungsgemeinschaft (BR5490/3)

Marko Brankatschk

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.