Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila

  1. Johanna Lattner
  2. Weihua Leng
  3. Elisabeth Knust
  4. Marko Brankatschk  Is a corresponding author
  5. David Flores-Benitez  Is a corresponding author
  1. Max-Planck Institute of Molecular Cell Biology and Genetics, Germany
  2. Technische Universität Dresden, Germany

Abstract

An efficient vectorial intracellular transport machinery depends on a well-established apico-basal polarity and is a prerequisite for the function of secretory epithelia. Despite extensive knowledge on individual trafficking pathways, little is known about the mechanisms coordinating their temporal and spatial regulation. Here, we report that the polarity protein Crumbs is essential for apical plasma membrane phospholipid-homeostasis and efficient apical secretion. Through recruiting βHeavy-Spectrin and MyosinV to the apical membrane, Crumbs maintains the Rab6-, Rab11- and Rab30-dependent trafficking and regulates the lipid phosphatases Pten and Ocrl. Crumbs knock-down results in increased apical levels of PI(4,5)P2 and formation of a novel, Moesin- and PI(4,5)P2-enriched apical membrane sac containing microvilli-like structures. Our results identify Crumbs as an essential hub required to maintain the organization of the apical membrane and the physiological activity of the larval salivary gland.

Data availability

We provide as "Source data" files all the data used for statistical analyses and generation of all graphs. These files are sorted according to the Figure and the corresponding supplemental figures, which correspond to Figure 1 and its supplements, Figure 2, Figure 3 and its supplements, Figure 5 and its supplements, and Figure 6

Article and author information

Author details

  1. Johanna Lattner

    Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3421-9134
  2. Weihua Leng

    Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    No competing interests declared.
  3. Elisabeth Knust

    Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Competing interests
    Elisabeth Knust, Reviewing editor, eLife.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2732-9135
  4. Marko Brankatschk

    The Biotechnological Center, Technische Universität Dresden, Dresden, Germany
    For correspondence
    marko.brankatschk@tu-dresden.de
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5274-4552
  5. David Flores-Benitez

    Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    For correspondence
    flores@mpi-cbg.de
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8244-9335

Funding

Max-Planck-Gesellschaft

  • Johanna Lattner
  • Weihua Leng
  • Elisabeth Knust
  • David Flores-Benitez

Deutsche Forschungsgemeinschaft (BR5490/2)

  • Marko Brankatschk

Deutsche Forschungsgemeinschaft (BR5490/3)

  • Marko Brankatschk

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Utpal Banerjee, University of California, Los Angeles, United States

Version history

  1. Received: August 6, 2019
  2. Accepted: October 25, 2019
  3. Accepted Manuscript published: November 7, 2019 (version 1)
  4. Version of Record published: November 27, 2019 (version 2)

Copyright

© 2019, Lattner et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Johanna Lattner
  2. Weihua Leng
  3. Elisabeth Knust
  4. Marko Brankatschk
  5. David Flores-Benitez
(2019)
Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila
eLife 8:e50900.
https://doi.org/10.7554/eLife.50900

Share this article

https://doi.org/10.7554/eLife.50900

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