(a) Diffusion signal intensity from measurements on spinal cords performed at 25° C after delipidation (n = 2) with 10% Triton X and after washing the Triton away. The mean (circles) and SD (shaded bands) of the attenuation are plotted for the delipidated samples (red) alongside pure aCSF (purple) and fixed undelipidated spinal cords (n = 6) (orange). (b) The initial attenuation of signal. Monoexponential fits of the attenuation from points 2–4 yielded 2.15 ± 0.02, 1.94 ± 0.02 and 0.87 ± 0.14 ×10−9 m2/s for the aCSF, delipidated, and undelipidated spinal cords and are shown as the dashed, solid, and dotted lines, respectively. (c) Diffusion coefficient distribution of the delipidated spinal cords, for which the mean (solid line) and SD (shaded band around line) are not significantly different from the pure aCSF (dashed line). The distribution from a fixed, undelipidated spinal cord (dotted line) is also shown for comparison. The purple, green, and yellow shading across the plots signifies water which is free, localized, and restricted.