Axon TRAP reveals learning-associated alterations in cortical axonal mRNAs in the lateral amgydala
- University of Connecticut, United States
- Karolinska Institutet, Sweden
- New York University, United States
- New York University School of Medicine, United States
Abstract
Local translation can support memory consolidation by supplying new proteins to synapses undergoing plasticity. Translation in adult forebrain dendrites is an established mechanism of synaptic plasticity and is regulated by learning, yet there is no evidence for learning-regulated protein synthesis in adult forebrain axons, which have traditionally been believed to be incapable of translation. Here we show that axons in the adult rat amygdala contain translation machinery, and use translating ribosome affinity purification (TRAP) with RNASeq to identify mRNAs in cortical axons projecting to the amygdala, over 1200 of which were regulated during consolidation of associative memory. Mitochondrial and translation-related genes were upregulated, whereas synaptic, cytoskeletal, and myelin-related genes were downregulated; the opposite effects were observed in the cortex. Our results demonstrate that axonal translation occurs in the adult forebrain and is altered after learning, supporting the likelihood that local translation is more a rule than an exception in neuronal processes.
Data availability
Sequencing data have been deposited in GEO under accession code GSE124592. All analyses are included in supporting files.
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The translatome of adult cortical neurons is regulated by learning in vivoNCBI Gene Expression Omnibus, GSE124592.
Article and author information
Author details
Funding
National Institute of Neurological Disorders and Stroke (NS034007)
- Eric Klann
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD082013)
- Eric Klann
National Institute of Mental Health (MH083583)
- Linnaea E Ostroff
National Institute of Neurological Disorders and Stroke (NS047384)
- Eric Klann
National Institute of Mental Health (MH094965)
- Linnaea E Ostroff
National Institute of Mental Health (MH119517)
- Linnaea E Ostroff
National Institute of Neurological Disorders and Stroke (NS087112)
- Emanuela Santini
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All animal procedures were performed in accordance with the guidelines in the National Institutes of Health Guide for the Care and Use of Laboratory Animals, and were approved by the Animal Care and Use Committees of New York University (protocol 01-1097) and the University of Connecticut (protocol A17-036).
Copyright
© 2019, Ostroff et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Axon TRAP reveals learning-associated alterations in cortical axonal mRNAs in the lateral amgydala
eLife 8:e51607.
https://doi.org/10.7554/eLife.51607
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