Representative images from intestinal cryosections stained with CD45-FITC, B220-PECF594 (red), Siglec F- PECF594 (yellow), CD3-PE (magenta), CD4-APC (green), CD64-PE (cyan) and DAPI (blue) from naïve (a) or day 14 hr. polygyrus infected (b) duodenal segments. Scale bar = 100 µm (representative of >10 sections from 3 to 5 mice per group from two independent experiments). (c) Quantification of CD45+, B220+, eosinophils (counted as non-epithelial Siglec F+ cells), CD3+ CD4+, CD3+ CD4- and CD64+ cells per villi section per mm2 (>15 villi from >10 sections from 3 to 5 mice per group from two independent experiments were quantified; unpaired t-test or 2-way ANOVA followed by Sidak’s multiple comparisons test compared to each of the naïve groups; **p≤0.01, ***p≤0.001). d, Comparison of immune cell frequencies detected by confocal microscopy (CM, as shown in c) or spectral flow cytometry (SFC, as shown in Figure 1a) from naïve or day 14 infected duodenal segments (mean ± s.e.m.; 2-way ANOVA followed by Sidak’s multiple comparisons test comparing detected cell frequencies between technologies or between naïve and infected segments; **p≤0.01, ***p≤0.001).