Yolk-sac-derived macrophages progressively expand in the mouse kidney with age
- Duke University School of Medicine, United States
Abstract
Renal macrophages represent a highly heterogeneous and specialized population of myeloid cells with mixed developmental origins from the yolk-sac and hematopoietic stem cells (HSC). They promote both injury and repair by regulating inflammation, angiogenesis, and tissue remodeling. Recent reports highlight differential roles for ontogenically distinct renal macrophage populations in disease. However, little is known about how these populations change over time in normal, uninjured kidneys. Prior reports demonstrated a high proportion of HSC-derived macrophages in the young adult kidney. Unexpectedly, using genetic fate-mapping and parabiosis studies, we found that yolk-sac-derived macrophages progressively expand in number with age and become a major contributor to the renal macrophage population in older mice. This chronological shift in macrophage composition involves local cellular proliferation and recruitment from circulating progenitors and may contribute to the distinct immune responses, limited reparative capacity, and increased disease susceptibility of kidneys in the elderly population.
Data availability
All data generated or analyzed during this study are included in the manuscript.
Article and author information
Author details
Funding
American Society of Nephrology (Career Developmental Grant)
- Tomokazu Souma
Duke University School of Medicine (Start-up Fund)
- Tomokazu Souma
National Institute on Aging (R01 AG049745)
- Benjamin A Alman
National Institute of Allergy and Infectious Diseases (AI088100)
- Mari L Shinohara
American Heart Association (Postdoctoral fellowship)
- Shintaro Ide
Kanzawa Medical Research Foundation (Fellowship)
- Yasuhito Yahara
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All animals were used according to the approved protocols (A051-18-02 and A196-16-0) by the Institutional Animal Care and Use Committee of Duke University.
Copyright
© 2020, Ide et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Yolk-sac-derived macrophages progressively expand in the mouse kidney with age
eLife 9:e51756.
https://doi.org/10.7554/eLife.51756
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