Pathway-, layer- and cell-type-specific thalamic input to mouse barrel cortex

  1. B Semihcan Sermet
  2. Pavel Truschow
  3. Michael Feyerabend
  4. Johannes M Mayrhofer
  5. Tess B Oram
  6. Ofer Yizhar
  7. Jochen F Staiger
  8. Carl CH Petersen  Is a corresponding author
  1. Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland
  2. Georg-August-University Goettingen, Germany
  3. Weizmann Institute of Science, Israel
8 figures, 5 tables and 1 additional file

Figures

Pathway-specific optogenetic stimulation of thalamus evokes stable monosynaptic input to neurons in whisker primary somatosensory barrel cortex (wS1).

(A) A Cre-ON (FLEX) AAV virus expressing tdTomato and a Cre-OFF (DFO) virus expressing YFP, were injected together into the thalamus of GPR26-Cre mice, which express Cre in the higher-order …

VPM input to excitatory neurons across layers in wS1.

(A) An example experiment (BS144) in which ChR2 was expressed in VPM and whole-cell recordings were obtained sequentially from 12 excitatory neurons across different cortical layers in wS1. Neurons …

POm input to excitatory neurons across layers in wS1.

(A) ChR2 was expressed in POm in the example experiment (BS087) and 10 excitatory neurons across different cortical layers in wS1 were recorded sequentially. Neurons were filled with biocytin and pos…

VPM input to GABAergic PV neurons across layers in wS1.

(A) An example experiment (BS265) in which ChR2 was expressed in VPM of a PV-Cre mouse crossed with a LSL-tdTomato report mouse, and whole-cell recordings were obtained sequentially neurons across …

POm input to GABAergic PV neurons across layers in wS1.

(A) An example experiment (BS271) in which ChR2 was expressed in POm and whole-cell recordings were obtained sequentially neurons across different cortical layers in wS1 of PV-Cre mice crossed with …

VPM and POm input to GABAergic SST neurons across layers in wS1.

(A) An example experiment (BS246) from a SST-Cre mouse crossed with a LSL-tdTomato reporter mouse, in which ChR2 was expressed in VPM and whole-cell recordings were obtained sequentially from …

VPM and POm input to GABAergic VIP neurons across layers in wS1.

(A) An example experiment (BS267) in which ChR2 was expressed in VPM of a VIP-Cre mouse crossed with a LSL-tdTomato reporter mouse, and whole-cell recordings were obtained sequentially from neurons …

Summary of layer-specific inputs from VPM and POm to distinct cell-types in wS1.

(A) The mean ± SEM of EPSP amplitudes across layers (normalized to L4 EXC) for excitatory neurons (black), PV neurons (red), SST neurons (brown) and VIP neurons (blue) (above). The equivalent plot …

Tables

Table 1
Normalized EPSP amplitudes of VPM input including: mean ± SD, median, and n = number of recorded cells.
VPM amplitudeEXCPVSSTVIP
L20.20 ± 0.54
0.022
n = 29
0.04 ± 0.02
0.032
n = 3
0.01 ± 0.00
0.0082
n = 3
0.13 ± 0.26
0.024
n = 10
L30.46 ± 0.50
0.26
n = 45
0.35 ± 0.34
0.19
n = 9
0.09 ± 0.10
0.025
n = 3
0.32 ± 0.49
0.046
n = 17
L41.00 ± 0.37
1.00
n = 65
1.15 ± 0.92
1.05
n = 14
0.09 ± 0.08
0.067
n = 8
0.15 ± 0.16
0.089
n = 8
L5A0.14 ± 0.21
0.063
n = 39
0.12 ± 0.22
0.016
n = 5
0.03 ± 0.01
0.021
n = 6
0.03 ± 0.01
0.029
n = 5
L5B0.17 ± 0.22
0.080
n = 44
0.04 ± 0.02
0.045
n = 11
0.01 ± 0.01
0.0073
n = 9
0.13 ± 0.23
0.028
n = 5
L60.39 ± 0.79
0.077
n = 40
0.17 ± 0.20
0.033
n = 5
0.01 ± 0.01
0.0060
n = 4
0.011
n = 1
Table 2
Normalized EPSP slopes of VPM input including: mean ± SD, median, and n = number of recorded cells.
VPM slopeEXCPVSSTVIP
L20.19 ± 0.50
0.018
n = 29
0.03 ± 0.03
0.021
n = 3
0.02 ± 0.01
0.016
n = 3
0.19 ± 0.35
0.021
n = 7
L30.51 ± 0.54
0.33
n = 45
0.85 ± 1.07
0.38
n = 9
0.03 ± 0.05
0.0049
n = 3
0.40 ± 0.61
0.038
n = 16
L41.00 ± 0.43
1.00
n = 65
2.73 ± 2.24
1.68
n = 14
0.06 ± 0.04
0.060
n = 8
0.09 ± 0.08
0.044
n = 8
L5A0.11 ± 0.16
0.043
n = 39
0.38 ± 0.76
0.0079
n = 5
0.01 ± 0.01
0.0061
n = 6
0.02 ± 0.02
0.0057
n = 5
L5B0.16 ± 0.23
0.060
n = 43
0.06 ± 0.04
0.077
n = 11
0.00 ± 0.00
0.0016
n = 6
0.16 ± 0.24
0.024
n = 4
L60.35 ± 0.81
0.037
n = 39
0.13 ± 0.16
0.015
n = 5
0.06 ± 0.05
0.082
n = 3
0.00072
n = 1
Table 3
Normalized EPSP amplitudes of POm input including: mean ± SD, median, and n = number of recorded cells.
POm amplitudeEXCPVSSTVIP
L20.11 ± 0.18
0.032
n = 38
0.17 ± 0.13
0.17
n = 3
0.02 ± 0.02
0.0066
n = 5
0.18 ± 0.37
0.036
n = 14
L30.16 ± 0.39
0.048
n = 31
0.04 ± 0.04
0.030
n = 7
0.07 ± 0.16
0.016
n = 7
0.17 ± 0.25
0.17
n = 20
L40.13 ± 0.35
0.011
n = 38
0.03 ± 0.06
0.013
n = 8
0.02 ± 0.01
0.034
n = 3
0.21 ± 0.35
0.067
n = 12
L5A1.00 ± 0.52
1.00
n = 75
1.17 ± 0.96
0.79
n = 8
0.15 ± 0.15
0.053
n = 5
0.33 ± 0.32
0.18
n = 10
L5B0.11 ± 0.31
0.026
n = 52
0.09 ± 0.10
0.015
n = 10
0.03 ± 0.04
0.015
n = 13
0.20 ± 0.18
0.18
n = 7
L60.23 ± 0.38
0.068
n = 47
0.03 ± 0.05
0.010
n = 10
0.01 ± 0.01
0.010
n = 18
0.090
n = 2
Table 4
Normalized EPSP slopes of POm input including: mean ± SD, median, and n = number of recorded cells.
POm slopeEXCPVSSTVIP
L20.08 ± 0.13
0.014
n = 38
0.31 ± 0.24
0.35
n = 3
0.02 ± 0.02
0.0080
n = 4
0.07 ± 0.10
0.043
n = 13
L30.30 ± 1.13
0.020
n = 31
0.09 ± 0.13
0.041
n = 5
0.17 ± 0.41
0.0024
n = 6
0.12 ± 0.12
0.12
n = 20
L40.32 ± 1.05
0.0045
n = 35
0.10 ± 0.26
0.0040
n = 8
0.01 ± 0.01
0.014
n = 3
0.14 ± 0.20
0.045
n = 12
L5A1.00 ± 0.56
1.00
n = 75
2.10 ± 1.96
1.25
n = 8
0.24 ± 0.34
0.045
n = 5
0.33 ± 0.40
0.16
n = 10
L5B0.13 ± 0.40
0.014
n = 50
0.12 ± 0.16
0.029
n = 10
0.04 ± 0.08
0.0090
n = 13
0.17 ± 0.18
0.16
n = 7
L60.29 ± 0.53
0.050
n = 44
0.05 ± 0.14
0.0040
n = 10
0.00 ± 0.00
0.00093
n = 14
0.015
n = 2
Key resources table
Reagent type
(species) or resource
DesignationSource or referenceIdentifiersAdditional information
Strain, strain background (Mus musculus)Short: Wild type
C57BL/6J
Jackson LaboratoryJAX:000664
Genetic reagent (Mus musculus)Short: GPR26-Cre
Tg(Gpr26-cre)
KO250Gsat/Mmucd
MMRRCRRID:
MMRRC_033032-UCD
(Gerfen et al., 2013)
Genetic reagent (Mus musculus)Short: PV-Cre
B6;129P2-Pvalbtm1(cre)Arbr/J
Jackson LaboratoryJAX:008069(Hippenmeyer et al., 2005)
Genetic reagent (Mus musculus)Short: Sst-Cre
Ssttm2.1(cre)Zjh/J
Jackson LaboratoryJAX:013044(Taniguchi et al., 2011)
Genetic reagent (Mus musculus)Short: VIP-Cre
Viptm1(cre)Zjh/J
Jackson LaboratoryJAX:010908(Taniguchi et al., 2011)
Genetic reagent (Mus musculus)Short: Gad2-Cre
Gad2tm2(cre)Zjh/J
Jackson LaboratoryJAX:010802(Taniguchi et al., 2011)
Genetic reagent (Mus musculus)Short: LSL-tdTomato
B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
Jackson LaboratoryJAX:007909(Madisen et al., 2010)
Recombinant DNA reagentAAV-FLEX-tdTomatoAddgeneAddgene #28306
Recombinant DNA reagentAAV-hSyn-ChR2-YFPU. PennAddgene #26973
Recombinant DNA reagentAAV-DIO-ChR2-YFPU. PennAddgene #20298
Recombinant DNA reagentAAV-DFO-ChR2-YFPThis paperAddgene #136916See Materials and methods Available from Addgene
Software, algorithmMatlab analysis code and dataThis paperZenodo doi: 10.5281/zenodo.3560697See Materials and methods
Available from https://zenodo.org/

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