ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein
- University of Pennsylvania, United States
- University of Pittsburgh, United States
- University of California, Irvine, United States
- Children's hospital of Philadelphia, United States
Abstract
Modulating cytoplasmic Ca2+ concentration ([Ca2+]i) by endoplasmic reticulum (ER)-localized inositol 1,4,5-trisphosphate receptor (InsP3R) Ca2+-release channels is a universal signaling pathway that regulates numerous cell-physiological processes. Whereas much is known regarding regulation of InsP3R activity by cytoplasmic ligands and processes, its regulation by ER-luminal Ca2+ concentration ([Ca2+]ER) is poorly understood and controversial. We discovered that the InsP3R is regulated by a peripheral membrane-associated ER-luminal protein that strongly inhibits the channel in the presence of high, physiological [Ca2+]ER. The widely-expressed Ca2+-binding protein annexin A1 (ANXA1) is present in the nuclear envelope lumen and, through interaction with a luminal region of the channel, can modify high-[Ca2+]ER inhibition of InsP3R activity. Genetic knockdown of ANXA1 expression enhanced global and local elementary InsP3-mediated Ca2+ signaling events. Thus, [Ca2+]ER is a major regulator of InsP3R channel activity and InsP3R-mediated [Ca2+]i signaling in cells by controlling an interaction of the channel with a peripheral membrane-associated Ca2+-binding protein, likely ANXA1.
Data availability
All data generated and analyzed are included in the manuscript and supporting files.
Article and author information
Author details
Karthik Mallilankaraman
Funding
National Institutes of Health (R37GM56328)
- J Kevin Foskett
National Institutes of Health (R01GM114042)
- Don-On Daniel Mak
National Institutes of Health (GM048071)
- Ian Parker
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Mark T Nelson, University of Vermont, United States
Version history
- Received: November 12, 2019
- Accepted: May 15, 2020
- Accepted Manuscript published: May 18, 2020 (version 1)
- Version of Record published: May 29, 2020 (version 2)
Copyright
© 2020, Vais et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein
eLife 9:e53531.
https://doi.org/10.7554/eLife.53531
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