Biocytin filled neurons were reconstructed using Neurolucida. (A) D-stellate cells (large cells) exhibited typical radiate morphology whereas (B) the smaller glycinergic cells show restricted axonal and dendritic arbors. (C) T-stellate cells show tuffed dendritic arbors and occupy regions parallel to the isofrequency bands. (D) Spread of axonal-dendritic arbors was quantified by measuring the longest and shortest axis of the reconstructed structures. Smaller glycinergic cells had significantly restricted axonal-dendritic process compared with D-stellate cells (longest axis: D-stellate cells, 619.42 ± 45.78 μm versus. small cells, 271.43 ± 26.27 μm, p<0.001, t-test; shorter axis: D-stellate cells, 435.62 ± 1.82 μm, n = 6 cells versus small cells, 148.60 ± 12.03 μm, n = 17, p<0.001, t-test). In comparison to T-stellate cells, small glycinergic cells had similar spread of the axonal-dendritic field in the longest axis but occupied a significantly larger region in the shortest axis longest axis: T-stellate cells, 237.03 ± 16.67 μm versus small cells, 271.43 ± 26.27 μm, p<0.33, t-test; shorter axis: T-stellate cells, 79.73 ± 13.68 μm versus small cells, 148.60 ± 12.03 μm, p<0.005, t-test. D- and T-stellate cells had significantly larger volume (volume: D-stellate cells, 4.8 ± 1.2×106 μm3 versus small cells, 2.02 ± 0.69×106 μm3, p<0.001, t-test) (E) and surface area (D-stellate cells, 20.91 ± 1.07×103 μm2 versus small cells, 9.83 ± 2.71 × 103 μm2, p<0.01, t-test) (F) compared with the small glycinergic cells. (F) Sholl analysis showing that small cells had significantly higher branching closer to soma compared with D- and T-stellate cells (Sholl analysis, p<0.05, K-S test).