Asymmetric clustering of centrosomes defines the early evolution of tetraploid cells
- Virginia Tech, United States
Abstract
Tetraploidy has long been of interest to both cell and cancer biologists, partly because of its documented role in tumorigenesis. A common model proposes that the extra centrosomes that are typically acquired during tetraploidization are responsible for driving tumorigenesis. However, tetraploid cells evolved in culture have been shown to lack extra centrosomes. This observation raises questions about how tetraploid cells evolve and more specifically about the mechanisms(s) underlying centrosome loss. Here, using a combination of fixed cell analysis, live cell imaging, and mathematical modeling, we show that populations of newly formed tetraploid cells rapidly evolve in vitro to retain a near-tetraploid chromosome number while losing the extra centrosomes gained at the time of tetraploidization. This appears to happen through a process of natural selection in which tetraploid cells that inherit a single centrosome during a bipolar division with asymmetric centrosome clustering are favored for long-term survival.
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All data generated during the study are provided in clearly labeled source data files in excel format.
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Author details
Funding
Virginia Tech College of Science (Dean's Discovery Fund)
- Daniela Cimini
Fralin Life Sciences Institute (Discretionary funds)
- Daniela Cimini
ICTAS Center for Engineered Health (Seed funding)
- Daniela Cimini
National Science Foundation (MCB-1517506)
- Daniela Cimini
Virginia Tech Graduate School (BIOTRANS IGEP)
- Nicolaas C Baudoin
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Baudoin et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Asymmetric clustering of centrosomes defines the early evolution of tetraploid cells
eLife 9:e54565.
https://doi.org/10.7554/eLife.54565
