Condensin I subunit Cap-G is essential for proper gene expression during the maturation of post-mitotic neurons
- Imperial College London, United Kingdom
- University of Bayreuth, Germany
Abstract
Condensin complexes are essential for mitotic chromosome assembly and segregation during cell divisions, however, little is known about their functions in post-mitotic cells. Here we report a role for the condensin I subunit Cap-G in Drosophila neurons. We show that, despite not requiring condensin for mitotic chromosome compaction, post-mitotic neurons express Cap-G. Knockdown of Cap-G specifically in neurons (from their birth onwards) results in developmental arrest, behavioural defects, and dramatic gene expression changes, including reduced expression of a subset of neuronal genes and aberrant expression of genes that are not normally expressed in the developing brain. Knockdown of Cap-G in mature neurons results in similar phenotypes but to a lesser degree. Furthermore, we see dynamic binding of Cap-G at distinct loci in progenitor cells and differentiated neurons. Therefore, Cap-G is essential for proper gene expression in neurons and plays an important role during the early stages of neuronal development.
Data availability
All raw sequence files and processed files have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus (accession number GSE142112).
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Characterisation of Cap-G binding and its role in post-mitotic neurons in DrosophilaGene Expression Omnibus, GSE142112.
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FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data.European Nucleotide Archive, PRJEB22205.
Article and author information
Author details
Funding
Wellcome (104567/Z/14/Z)
- Tony D Southall
Biotechnology and Biological Sciences Research Council (BB/P017924/1)
- Gabriel N Aughey
- Tony D Southall
Biotechnology and Biological Sciences Research Council (BB/M011178/1)
- Amira Hassan
Deutsche Forschungsgemeinschaft (HE2354/23-2)
- Stefan K Heidmann
Deutsche Forschungsgemeinschaft (HE2354/4-1)
- Stefan K Heidmann
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Anne E West, Duke University School of Medicine, United States
Version history
- Received: January 14, 2020
- Accepted: April 6, 2020
- Accepted Manuscript published: April 7, 2020 (version 1)
- Version of Record published: April 20, 2020 (version 2)
Copyright
© 2020, Hassan et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Condensin I subunit Cap-G is essential for proper gene expression during the maturation of post-mitotic neurons
eLife 9:e55159.
https://doi.org/10.7554/eLife.55159
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