LTD at amygdalocortical synapses as a novel mechanism for hedonic learning
- Department of Neurobiology and Behavior, SUNY – Stony Brook, United States
Figures
Figure 1 with 2 supplements
Conditioned taste aversion learning differentially recruits EXC neurons in L2/3 aGC.
(A) Behavioral paradigm schematic. Animals in the control group (N = 30) received ip LiCl (7.5 mL/kg of 0.15M LiCl) the evening prior to exposure to a sucrose solution. Animals in the CTA group (N = …
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Figure 1—source data 1
This file contains source data and statistics presented in each panel of Figure 1, Figure 1—figure supplement 1 and Figure 1—figure supplement 2.
Excel worksheet tabs are labeled to indicate the specific figure. Each data table is labeled to indicate the specific figure panel. The statistics worksheet indicates specific tests and values applied to each data set in the figure and supplements.
- https://cdn.elifesciences.org/articles/55175/elife-55175-fig1-data1-v1.xlsx
Figure 1—figure supplement 1
Baseline water consumption and initial sucrose palatability do not differ between groups.
(A) Average water consumption prior to conditioning did not differ between groups and rats in both groups showed an initial preference for sucrose over water (Control, [N = 30]: average H2O 4.42 ± 0.…
Figure 1—figure supplement 2
Stability of CTA learning across testing days.
(A) Diagram of behavioral paradigm to assess whether the 2-bottle test represents an extinction trial. (B) Average fluid consumption across training sessions (Extinction testing, [N = 8 rats]: …
Figure 2 with 1 supplement
CTA learning does not affect intrinsic excitability but reduces excitatory synaptic drive onto EXC neurons in L2/3 aGC.
(A) Images of example recorded neurons from a control animal (top) and CTA animal (bottom). Left: biocytin (green), middle: GAD-67 (magenta), right: Merge showing lack of co-localization. Scale bar =…
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Figure 2—source data 1
This file contains source data and statistics presented in each panel of Figure 2, Figure 2—figure supplement 1.
Excel worksheet tabs are labeled to indicate the specific figure. Each data table is labeled to indicate the specific figure panel. The statistics worksheet indicates specific tests and values applied to each data set in the figure and supplements.
- https://cdn.elifesciences.org/articles/55175/elife-55175-fig2-data1-v1.xlsx
Figure 2—figure supplement 1
Changes in synaptic drive do not mask changes in intrinsic excitability.
(A) FI curves from the control group before and after bath application of synaptic blockers (20 μm DNQX, 50 μm AP5, 20 μm picrotoxin). Average FI slope decreased (Control [n = 18 cells from three …
Figure 3 with 1 supplement
CTA decreases BLA input onto L2/3 pyramidal neurons in aGC.
(A) Example of histological verification of injection site and expression of opsin construct in BLA terminal fields in aGC. Left: Image of ChR2-Venus expression at BLA injection site (scale bar = 500…
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Figure 3—source data 1
This file contains source data and statistics presented in each panel of Figure 3, Figure 3—figure supplement 1.
Excel worksheet tabs are labeled to indicate the specific figure. Each data table is labeled to indicate the specific figure panel. The statistics worksheet indicates specific tests and values applied to each data set in the figure and supplements.
- https://cdn.elifesciences.org/articles/55175/elife-55175-fig3-data1-v1.xlsx
Figure 3—figure supplement 1
CTA does not affect connectivity, PPR, CV, or decay tau.
(A) CTA does not affect the percentage of neurons that receive input from BLA (Control [N = 16 rats] 72.43 ± 14.19% vs. CTA [N = 13 rats] 69.74 ± 15.04%, p=0.80), paired-pulse ratio (Control [N = 13 …
Figure 4 with 1 supplement
CTA learning occludes LTD induction onto L2/3 pyramidal neurons in aGC.
(A) Schematic of 20 Hz plasticity induction paradigm. BLA terminal fields were activated with 20 bursts of 20 light pulses (5 ms) at 20 Hz delivered every 250 ms, while the postsynaptic neuron was …
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Figure 4—source data 1
This file contains source data and statistics presented in each panel of Figure 4, Figure 4—figure supplement 1.
Excel worksheet tabs are labeled to indicate the specific figure. Each data table is labeled to indicate the specific figure panel. The statistics worksheet indicates specific tests and values applied to each data set in the figure and supplements.
- https://cdn.elifesciences.org/articles/55175/elife-55175-fig4-data1-v1.xlsx
Figure 4—figure supplement 1
Activity during 20Hz induction protocol and relationship between baseline PPR, CV, or decay tau and post-induction plasticity.
(A) Sample traces from the control and CTA group before and after plasticity induction superimposed. (B) Summary of spiking activity during the 20 Hz plasticity induction protocol. In both groups, …
Figure 5 with 1 supplement
Occlusion of LTD following CTA is specific to BLA activity regime.
(A) Schematic of tonic induction paradigm. BLA terminal fields were activated with 6 s ramping stimuli delivered every 250 ms, while the postsynaptic neuron was depolarized subthreshold by injection …
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Figure 5—source data 1
This file contains source data and statistics presented in each panel of Figure 5, Figure 5—figure supplement 1.
Excel worksheet tabs are labeled to indicate the specific figure. Each data table is labeled to indicate the specific figure panel. The statistics worksheet indicates specific tests and values applied to each data set in the figure and supplements.
- https://cdn.elifesciences.org/articles/55175/elife-55175-fig5-data1-v1.xlsx
Figure 5—figure supplement 1
Activity during ramp induction protocol and relationship between baseline PPR, CV, or decay tau and post-induction plasticity.
(A) Sample traces from the control and CTA group before and after plasticity induction superimposed. (B) Summary of spiking activity during the ramp plasticity induction protocol. The ramp induction …
Figure 6 with 1 supplement
BLA terminal field activation in vivo with the phasic 20 Hz pattern eliminated sucrose preference.
(A) Cartoon of experimental approach. AAV9.hSyn.eGFP.WPRE.bGH: (GFP control group) or AAV9.CAG.ChR2-Venus.WPRE.SV40 (Paired 20 Hz group) were injected in BLA and an optic fiber (400 µm diameter, …
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Figure 6—source data 1
This file contains source data and statistics presented in each panel of Figure 6, Figure 6—figure supplement 1.
Excel worksheet tabs are labeled to indicate the specific figure. Each data table is labeled to indicate the specific figure panel. The statistics worksheet indicates specific tests and values applied to each data set in the figure and supplements.
- https://cdn.elifesciences.org/articles/55175/elife-55175-fig6-data1-v1.xlsx
Figure 6—figure supplement 1
Baseline water consumption and initial sucrose palatability do not differ between groups.
(A) Average water consumption prior to conditioning did not differ between groups (GFP control: average H2O 6.87 ± 0.41 mL/day vs. paired 20 Hz opto: average H2O 7.23 ± 0.42 mL/day vs. non-paired 20 …
