1. Neuroscience
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Spatiotemporal dynamics of multi-vesicular release is determined by heterogeneity of release sites in central synapses

  1. Dario Maschi
  2. Vitaly Klyachko  Is a corresponding author
  1. Washington University, United States
Research Article
  • Cited 5
  • Views 1,852
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Cite this article as: eLife 2020;9:e55210 doi: 10.7554/eLife.55210

Abstract

Synaptic active zone (AZ) can release multiple vesicles in response to an action potential. This multi-vesicular release (MVR) occurs at most synapses, but its spatiotemporal properties are unknown. Nanoscale-resolution detection of individual release events in hippocampal synapses revealed unprecedented heterogeneity among vesicle release sites within a single AZ, with a gradient of release probability decreasing from AZ center to periphery. Parallel to this organization, MVR events preferentially overlap with uni-vesicular release (UVR) events at sites closer to AZ center. Pairs of fusion events comprising MVR are also not perfectly synchronized, and the earlier event tends to occur closer to AZ center. The spatial features of release sites and MVR events are similarly tightened by buffering intracellular calcium. These observations revealed a marked heterogeneity of release site properties within individual AZs, which determines the spatiotemporal features of MVR events and is controlled, in part, by non-uniform calcium elevation across the AZ.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files

Article and author information

Author details

  1. Dario Maschi

    Department of Cell Biology and Physiology, Washington University, St Louis, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Vitaly Klyachko

    Department of Cell Biology and Physiology, Washington University, St Louis, United States
    For correspondence
    klyachko@wustl.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3449-243X

Funding

National Institute of Neurological Disorders and Stroke (NS111596)

  • Vitaly Klyachko

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: All animal procedures conformed to the guidelines approved by the Washington University Animal Studies Committee (protocol approval # 20170233).

Reviewing Editor

  1. Karel Svoboda, Janelia Research Campus, Howard Hughes Medical Institute, United States

Publication history

  1. Received: January 16, 2020
  2. Accepted: January 28, 2020
  3. Accepted Manuscript published: February 6, 2020 (version 1)
  4. Version of Record published: March 6, 2020 (version 2)

Copyright

© 2020, Maschi & Klyachko

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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