A whole-brain connectivity map of mouse insular cortex
- Max-Planck Institute of Neurobiology, Germany
- Max von Pettenkofer-Institute & Gene Center, Germany
Abstract
The insular cortex (IC) plays key roles in emotional and regulatory brain functions and is affected across psychiatric diseases. However, the brain-wide connections of the mouse IC have not been comprehensively mapped. Here we traced the whole-brain inputs and outputs of the mouse IC across its rostro-caudal extent. We employed cell-type specific monosynaptic rabies virus tracings to characterize afferent connections onto either excitatory or inhibitory IC neurons, and adeno-associated viral tracings to label excitatory efferent axons. While the connectivity between the IC and other cortical regions was highly bidirectional, the IC connectivity with subcortical structures was often unidirectional, revealing prominent cortical-to-subcortical or subcortical-to-cortical pathways. The posterior and medial IC exhibited resembling connectivity patterns, while the anterior IC connectivity was distinct, suggesting two major functional compartments. Our results provide insights into the anatomical architecture of the mouse IC and thus a structural basis to guide investigations into its complex functions.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided in Supplementary File 2.
Article and author information
Author details
Funding
Max-Planck-Gesellschaft
- Caroline Weiand
- Nadine Gogolla
Deutsche Forschungsgemeinschaft (SPP1665)
- Daniel August Gehrlach
- Alexandru A Hennrich
- Karl-Klaus Conzelmann
- Nadine Gogolla
Horizon 2020 Framework Programme (ERC-2017-STG 758448)
- Thomas N Gaitanos
- Nadine Gogolla
Agence Nationale de la Recherche (ANR-17-CE37-0021)
- Alexandra S Klein
- Nadine Gogolla
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Kate M Wassum, University of California, Los Angeles, United States
Ethics
Animal experimentation: All animals were used in accordance with the regulations and under licenses obtained from the government of Upper Bavaria (Animal license AZ: 55.2-1-54-2532-56-2014).
Version history
- Received: January 29, 2020
- Accepted: September 16, 2020
- Accepted Manuscript published: September 17, 2020 (version 1)
- Version of Record published: October 6, 2020 (version 2)
Copyright
© 2020, Gehrlach et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A whole-brain connectivity map of mouse insular cortex
eLife 9:e55585.
https://doi.org/10.7554/eLife.55585
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