We report improved automated open-source methodology for head-fixed mesoscale cortical imaging and/or behavioral training of home cage mice using Raspberry Pi-based hardware. Staged partial and probabilistic restraint allows mice to adjust to self-initiated headfixation over 3 weeks' time with ~50% participation rate. We support a cue-based behavioral licking task monitored by a capacitive touch-sensor water spout. While automatically head-fixed, we acquire spontaneous, movement-triggered, or licking task-evoked GCaMP6 cortical signals. An analysis pipeline marked both behavioral events, as well as analyzed brain fluorescence signals as they relate to spontaneous and/or task-evoked behavioral activity. Mice were trained to suppress licking and wait for cues that marked the delivery of water. Correct rewarded go-trials were associated with widespread activation of midline and lateral barrel cortex areas following a vibration cue and delayed frontal and lateral motor cortex activation. Cortical GCaMP signals predicted trial success and correlated strongly with trial-outcome dependent body movements.
The name of each brain imaging file which contains both the mouse ID and the time stamp can be found in the SQL database (RFIDtag_xxxx_timestamp.raw; see Methods for URL and hosted as a full text file archive on Zenodo (https://doi.org/10.5281/zenodo.3268838) for the 5 cages of male mice that compose figures 1-7 and cage 6 female mice https://doi.org/10.5683/SP2/9RFXRP.All text file behavioral data is included online as well as image data for figures 8 and supplemental figure 2 are found on https://doi.org/10.5281/zenodo.3243572, all data files and code for figures 9 and 10 are found in https://doi.org/10.5683/SP2/ZTOPUM and female mouse behavioral data https://doi.org/10.5683/SP2/9RFXRP. All Python data acquisition code can be found on https://github.com/jamieboyd/AutoHeadFix/ and https://github.com/ubcbraincircuits/AutoHeadFix.
Homecage data for figures 8 and 9 and behavioral databaseZenodo 3243572.
Code and .mat files for automated homecage paper (Figs 9 and 10)https://doi.org/10.5683/SP2/ZTOPUM.
Home cage data female mice cage 6 all text file and database informationhttps://doi.org/10.5683/SP2/9RFXRP.
- Timothy H Murphy
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Animal experimentation: All procedures were conducted with approval from the University of British Columbia Animal Care Committee and in accordance with guidelines set forth by the Canadian Council for Animal Care.
- David Kleinfeld, University of California, San Diego, United States
© 2020, Murphy et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.