Metaphyseal cortical porosity in female and male control (Dmp1Cre) and Dmp1Cre:Il6stf/f mice (A) and Dmp1Cre:Socs3f/f and Dmp1Cre:Socs3f/f:Il6stf/f mice (B) and representative Otsu-based pseudocolorised images (C). (D) Bone volume, as a percentage of total metaphyseal volume, segregated by low, mid and high density volumes, in 12 week old Dmp1Cre:Socs3f/f and Dmp1Cre:Socs3f/f:Il6stf/f mice. Changes in low-, mid- and high-density bone are indicated by coloured asterisks; error bars shown are SEM for the low-, mid- and high-density bone volumes. *, p<0.5; **, p<0.01; ***, p<0.001 for comparisons shown, determined by repeated measures two-way ANOVA with Šidák post-hoc test; n = 9–11 mice per group. (E–H) Bone volume at low-, mid-, and high-density mineral levels in each slice from the distal to proximal end of the metaphyseal region in 12 week old female (E,F) and male (G,H) Dmp1Cre:Socs3f/f femora (E,G) and Dmp1Cre:Socs3f/f:Il6stf/f (F,H) f. Values are mean+ SEM, n = 9–11 mice per group; arrows denote where data becomes significantly different to the furthest slice from the arrow; colours denote the density level of bone that has changed. Pseudo-colourised images show a representative sample for the top and bottom slice for each graph. Differences between genotypes are shown in Figure 5—figure supplement 1 .