Aversive stimuli bias corticothalamic responses to motivationally significant cues
- The Solomon H Snyder Department of Neuroscience, Brain Science Institute, Kavli Neuroscience Discovery Institute, The Johns Hopkins University School of Medicine, United States
Figures
Figure 1 with 1 supplement
Behavioral responses to motivationally-significant predictive cues are modulated by history of aversive stimuli.
(A) Task design and experimental timeline. During ten conditioning sessions, odors (A, B, C, and D) predicted an appetitive sucrose solution, an aversive denatonium solution, no outcome, and either …
Figure 1—figure supplement 1
Mean ± SEM licking rates during anticipation periods (CS and delay), for mice exposed (orange) or unexposed (black) to air puffs.
Left: first probe (Day 11). Right: second probe after reversal (Day 22).
Figure 2 with 1 supplement
Neuronal responses across learning.
(A) Firing rates of neurons in mice unexposed (top) and exposed (bottom) to air puffs relative to pre-CS firing rates. Histogram scale bars: 700 neurons (top), 500 neurons (bottom). (B) …
Figure 2—figure supplement 1
Drawings illustrate recording sites in mPFC in no air puff (left) and air puff exposed (right) mice.
Boxes indicate approximate location of recording sites in each mouse, taking into account any vertical distance traveled during training and the approximate lateral spread of the tetrode bundle.
Figure 3
Aversive stimuli increase mPFC firing rates to motivationally relevant cues.
(A–B) Average firing rates (top) and histograms of firing rates during odor and delay period (bottom) from example neurons in a mouse unexposed (A) and exposed (B) to air puffs. Red: denatonium …
Figure 4 with 1 supplement
Air-puff-predicting stimuli modulate mPFC→PVT neuron firing rates.
(A) Schematic drawings of viral stereotaxic injection of AAV1-CaMKII-ChR2-eYFP and tetrode bundle into mPFC and optic fiber over PVT. (B) eYFP (green), and DAPI (blue) in mPFC (top) and PVT (bottom) …
Figure 4—figure supplement 1
Electrode and optic fiber locations.
(A) Drawings illustrate recording sites in mPFC in no air puff (left, ) and air puff exposed (right, ) mice. Boxes indicate approximate location of recording sites in each mouse, taking into …
Figure 5
Aversive stimuli increase corticothalamic firing rates to motivationally-relevant cues.
(A–B) Average firing rates (left) and histograms of firing rates during odor and delay period (right) from example neurons in a mouse unexposed (A) and exposed (B) to air puffs. Red: denatonium …
Figure 6 with 2 supplements
Optogenetic excitation of corticothalamic neurons negatively biases responses to motivationally-relevant stimuli.
(A) Schematic of viral stereotaxic injection of AAV1/5-CaMKIIa-ChR2-eYFP or AAV1/5-CaMKIIa-eYFP into mPFC and optic fiber over PVT. (B) Optical stimulation was delivered during presentation of the …
Figure 6—figure supplement 1
Virus expression and optic fiber locations.
(A) eYFP (green) and DAPI (blue) expression in a mPFC (left) and PVT (right) coronal section from a BL6 mouse that received AAV1-CaMKIIα-Halo-eYFP into mPFC and an optic fiber over PVT (scale bar, …
Figure 6—figure supplement 2
mPFC→PVT stimulation did not suppress licking for unexpected rewards.
Left: optical stimulation was delivered during the presentation of reward delivery and lasted for 1500 ms, Right: licking behavior in 10 Hz ChR2-eYFP (left) and 20 Hz ChR2-eYFP (right) groups across …
Figure 7
Optogenetic inhibition of corticothalamic neurons prevents negatively bias responses to motivationally-relevant stimuli.
(A) Schematic of viral stereotaxic injection of AAV1-CaMKIIa-eNpHR3.0-EYFP or AAV1-CaMKIIa-EYFP into mPFC and optic fiber over PVT. (B) Optical stimulation was delivered during presentation of the …
