(a) Loss-of-function in sax-3 causes defects in AMsh glial migration. Confocal images and schematic representation of AMsh glia in control and sax-3(yad10) animals expressing Pf53f4.13::GFP (yadIs48)…
(a) Diagram shows the mutations in four different sax-3 alleles examined in this study. (b) Data showing the distance between the nose tip and the center of AMsh cell bodies at different …
(a) SAX-3 is cleaved in the extracellular region during development. A schematic diagram at the top panel shows that the HA tag was fused at the C-terminal of SAX-3, and the leftover of the …
Images show the results from a second anti-HA antibody to confirm that SAX-3 has a developmental-stage-dependent cleavage (a), and this cleavage does not depend on slt-1 (b). (c) Deletion of the …
(a) Image from western blot show the SAX-3 extracellular fragment by N-terminal tagging FLAG transgenes. Stars (*) label bands that are nonspecifically recognized by the anti-FLAG antibody. A …
(a) A image from silver staining shows the full length (black stars) and cleaved fragment (red stars) of SAX-3. These samples were then submitted for proteomics. (b) A screenshot of the Scaffold …
(a) Confocal images show that a 2.5 kb syg-1 promoter can drive GFP expression in AMsh (labeled by Pf53f4.13::H2B::mCherry) in embryos (left) and larva (right). Scale bar, 10 µm. (b) Expression of sy…
Strains and plasmids used in this study.
A list of protein identified by proteomics show specific interactions with SAX-3 in 1.5- to 3-fold stage embryos.