Robo functions as an attractive cue for glial migration through SYG-1/Neph
- Department of Molecular Genetics and Microbiology, Duke University Medical Center, United States
- Department of Neurobiology, Regeneration Next Initiative, Duke Center for Neurodegeneration and Neurotherapeutics, and Duke Institute for Brain Sciences, Duke University Medical Center, United States
Figures
Figure 1 with 1 supplement
sax-3/Robo regulates AMsh glial migration in a Slit-independent fashion.
(a) Loss-of-function in sax-3 causes defects in AMsh glial migration. Confocal images and schematic representation of AMsh glia in control and sax-3(yad10) animals expressing Pf53f4.13::GFP (yadIs48)…
Figure 1—figure supplement 1
Loss-of-function of sax-3/Robo causes AMsh migration defects.
(a) Diagram shows the mutations in four different sax-3 alleles examined in this study. (b) Data showing the distance between the nose tip and the center of AMsh cell bodies at different …
Figure 2 with 1 supplement
The extracellular cleavage of SAX-3 is required for AMsh glial migration.
(a) SAX-3 is cleaved in the extracellular region during development. A schematic diagram at the top panel shows that the HA tag was fused at the C-terminal of SAX-3, and the leftover of the …
Figure 2—figure supplement 1
The Slit-independent SAX-3 cleavage.
Images show the results from a second anti-HA antibody to confirm that SAX-3 has a developmental-stage-dependent cleavage (a), and this cleavage does not depend on slt-1 (b). (c) Deletion of the …
Figure 3 with 2 supplements
SYG-1 functions as a receptor for the SAX-3 extracellular cleavage fragment during AMsh glial migration.
(a) Image from western blot show the SAX-3 extracellular fragment by N-terminal tagging FLAG transgenes. Stars (*) label bands that are nonspecifically recognized by the anti-FLAG antibody. A …
Figure 3—figure supplement 1
Identification of SAX-3 interacting proteins by proteomics.
(a) A image from silver staining shows the full length (black stars) and cleaved fragment (red stars) of SAX-3. These samples were then submitted for proteomics. (b) A screenshot of the Scaffold …
Figure 3—figure supplement 2
SYG-1 is expressed in AMsh glia.
(a) Confocal images show that a 2.5 kb syg-1 promoter can drive GFP expression in AMsh (labeled by Pf53f4.13::H2B::mCherry) in embryos (left) and larva (right). Scale bar, 10 µm. (b) Expression of sy…
Figure 4
A model for regulation of AMsh glial migration by SAX-3 and SYG-1.
Additional files
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Supplementary file 1
Strains and plasmids used in this study.
- https://cdn.elifesciences.org/articles/57921/elife-57921-supp1-v1.docx
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Supplementary file 2
A list of protein identified by proteomics show specific interactions with SAX-3 in 1.5- to 3-fold stage embryos.
- https://cdn.elifesciences.org/articles/57921/elife-57921-supp2-v1.xlsx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/57921/elife-57921-transrepform-v1.docx
