Robo functions as an attractive cue for glial migration through SYG-1/Neph

  1. Zhongwei Qu
  2. Albert Zhang
  3. Dong Yan  Is a corresponding author
  1. Department of Molecular Genetics and Microbiology, Duke University Medical Center, United States
  2. Department of Neurobiology, Regeneration Next Initiative, Duke Center for Neurodegeneration and Neurotherapeutics, and Duke Institute for Brain Sciences, Duke University Medical Center, United States
4 figures and 3 additional files

Figures

Figure 1 with 1 supplement
sax-3/Robo regulates AMsh glial migration in a Slit-independent fashion.

(a) Loss-of-function in sax-3 causes defects in AMsh glial migration. Confocal images and schematic representation of AMsh glia in control and sax-3(yad10) animals expressing Pf53f4.13::GFP (yadIs48)…

Figure 1—figure supplement 1
Loss-of-function of sax-3/Robo causes AMsh migration defects.

(a) Diagram shows the mutations in four different sax-3 alleles examined in this study. (b) Data showing the distance between the nose tip and the center of AMsh cell bodies at different …

Figure 2 with 1 supplement
The extracellular cleavage of SAX-3 is required for AMsh glial migration.

(a) SAX-3 is cleaved in the extracellular region during development. A schematic diagram at the top panel shows that the HA tag was fused at the C-terminal of SAX-3, and the leftover of the …

Figure 2—figure supplement 1
The Slit-independent SAX-3 cleavage.

Images show the results from a second anti-HA antibody to confirm that SAX-3 has a developmental-stage-dependent cleavage (a), and this cleavage does not depend on slt-1 (b). (c) Deletion of the …

Figure 3 with 2 supplements
SYG-1 functions as a receptor for the SAX-3 extracellular cleavage fragment during AMsh glial migration.

(a) Image from western blot show the SAX-3 extracellular fragment by N-terminal tagging FLAG transgenes. Stars (*) label bands that are nonspecifically recognized by the anti-FLAG antibody. A …

Figure 3—figure supplement 1
Identification of SAX-3 interacting proteins by proteomics.

(a) A image from silver staining shows the full length (black stars) and cleaved fragment (red stars) of SAX-3. These samples were then submitted for proteomics. (b) A screenshot of the Scaffold …

Figure 3—figure supplement 2
SYG-1 is expressed in AMsh glia.

(a) Confocal images show that a 2.5 kb syg-1 promoter can drive GFP expression in AMsh (labeled by Pf53f4.13::H2B::mCherry) in embryos (left) and larva (right). Scale bar, 10 µm. (b) Expression of sy…

A model for regulation of AMsh glial migration by SAX-3 and SYG-1.

Additional files

Supplementary file 1

Strains and plasmids used in this study.

https://cdn.elifesciences.org/articles/57921/elife-57921-supp1-v1.docx
Supplementary file 2

A list of protein identified by proteomics show specific interactions with SAX-3 in 1.5- to 3-fold stage embryos.

https://cdn.elifesciences.org/articles/57921/elife-57921-supp2-v1.xlsx
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https://cdn.elifesciences.org/articles/57921/elife-57921-transrepform-v1.docx

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