Light-controlled engagement of microglia to focally remodel synapses in the adult brain
- Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE), Germany
- Institute of Biochemistry and Cell Biology, Germany
Abstract
Microglia continuously monitor synapses, but active synaptic remodelling by microglia in mature healthy brains is rarely directly observed. We performed targeted photoablation of single synapses in mature transgenic mice expressing fluorescent labels in neurons and microglia. The photodamage focally increased the duration of microglia-neuron contacts, and dramatically exacerbated both the turnover of dendritic spines and presynaptic boutons as well as the generation of new filopodia originating from spine heads or boutons. The results of microglia depletion confirmed that elevated spine turnover and the generation of presynaptic filopodia are microglia-dependent processes.
Data availability
All measurements, statistical analyses and the R code generated and used in this study are included in the manuscript.
Article and author information
Author details
Alexander Dityatev
Funding
The federal state Saxony-Anhalt and the European Structural and Investment Funds (ZS/2016/08/80645)
- Klaus-Dieter Fischer
- Alexander Dityatev
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All animals were treated in strict accordance with ethical animal research standards defined by the Directive 2010/63/EU, German law and approved by the Ethical Committee on Animal Health and Care of Saxony-Anhalt state, Germany (license number: 42502-2-1346).
Copyright
© 2020, Cangalaya et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Light-controlled engagement of microglia to focally remodel synapses in the adult brain
eLife 9:e58435.
https://doi.org/10.7554/eLife.58435
Further reading
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