A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast

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Abstract

In fission yeast, the septation initiation network (SIN) ensures temporal coordination between actomyosin ring (CAR) constriction with membrane ingression and septum synthesis. However, questions remain about CAR regulation under stress conditions. We show that Rgf1p (Rho1p GEF), participates in a delay of cytokinesis under cell wall stress (blankophor, BP). BP did not interfere with CAR assembly or the rate of CAR constriction, but did delay the onset of constriction in the wild type cells but not in the rgf1Δ cells. This delay was also abolished in the absence of Pmk1p, the MAPK of the cell integrity pathway (CIP), leading to premature abscission and a multi-septated phenotype. Moreover, cytokinesis delay correlates with maintained SIN signaling and depends on the SIN to be achieved. Thus, we propose that the CIP participates in a checkpoint, capable of triggering a CAR constriction delay through the SIN pathway to ensure that cytokinesis terminates successfully.

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All data generated or analysed during this study are included in the manuscript and supporting files

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Tomás Edreira

Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain

Competing interests
The authors declare that no competing interests exist.
Rubén Celador

Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain

Competing interests
The authors declare that no competing interests exist.
Elvira Manjón

Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain

Competing interests
The authors declare that no competing interests exist.
Yolanda Sánchez

Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain

For correspondence
ysm@usal.es

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-7650-9319

Funding

Regional Governmen of Castile and Leon co-financed by the European Social Fund

Tomás Edreira
Elvira Manjón

University of Salamanca

Rubén Celador

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.