1. Cell Biology

PLK-1 promotes the merger of the parental genome into a single nucleus by triggering lamina disassembly

  1. Griselda Velez-Aguilera
  2. Sylvia Nkombo Nkoula
  3. Batool Ossareh-Nazari
  4. Jana Link
  5. Dimitra Paouneskou
  6. Lucie Van Hove
  7. Nicolas Joly
  8. Nicolas Tavernier
  9. Jean-Marc Verbavatz
  10. Verena Jantsch
  11. Lionel Pintard  Is a corresponding author
  1. Programme Equipe Labéllisée Ligue Contre le Cancer - Team Cell Cycle & Development - Université de Paris, CNRS, Institut Jacques Monod, France
  2. Department of Chromosome Biology, Max Perutz Laboratories, University of Vienna, Vienna Biocenter, Austria
  3. Université de Paris, CNRS, Institut Jacques Monod, France
https://doi.org/10.7554/eLife.59510
Share this article
Cite this article
  1. Griselda Velez-Aguilera
  2. Sylvia Nkombo Nkoula
  3. Batool Ossareh-Nazari
  4. Jana Link
  5. Dimitra Paouneskou
  6. Lucie Van Hove
  7. Nicolas Joly
  8. Nicolas Tavernier
  9. Jean-Marc Verbavatz
  10. Verena Jantsch
  11. Lionel Pintard
(2020)
PLK-1 promotes the merger of the parental genome into a single nucleus by triggering lamina disassembly
eLife 9:e59510.
https://doi.org/10.7554/eLife.59510
  2. Download BibTeX
  3. Download .RIS
7 figures, 1 table and 5 additional files

Figures

Figure 1 with 1 supplement
Download asset Open asset
PLK-1 phosphorylates LMN-1 head and tail domains at multiple sites in vitro.

(A) Schematic of LMN-1 domain structure. Similar to lamins from other species, LMN-1 has a tripartite structure consisting of non-α-helical N-terminal head (violet) and C-terminal tail (blue) domains flanking an α-helical central rod domain. The C-terminal tail contains the Ig-fold motif. (B) Schematic of the approach used to investigate whether PLK-1 directly phosphorylates LMN-1 head and tail domains and to map the phosphorylated sites by tandem mass spectrometry (LC-MS/MS). (C) In vitro kinase assay was performed with C. e PLK-1 and the GST-LMN-1 head or tail as substrates. The samples were subjected to Phos-Tag SDS-PAGE followed by a western blot analysis using GST antibody. (D–E) Protein sequence of the head and tail domains with the residues phosphorylated by PLK-1 in red. The position of the phosphorylated residues is also indicated by white circles. The different non-phosphorylatable versions of LMN-1[H] 3A, 4A, 7A, 9A and LMN-1[T1] 4A, 6A with the positions of the alanine substitutions are presented below the protein sequence. (F–G) In vitro kinase assays using PLK-1 and LMN-1 WT or mutated fragments (LMN-1[H] and [T1]) as substrates. An autoradiograph of the SDS-PAGE showing γ-[32P] incorporation in LMN-1 (upper panel). Coomassie brilliant blue (CBB) staining of the same SDS-PAGE (bottom panel). Asterisk marks autophosphorylated PLK-1. GST is present as an impurity from the production of GST-LMN-1 fragments. (H–I) The graphs correspond to the quantification of the radioactivity incorporated into LMN-1 [H] and [T1] fragments divided by the total amount of LMN-1 fragments quantified using tryptophan fluorescence (Stain-Free; Bio-Rad). The ratio obtained for the WT fragments was arbitrary defined as 100. The quantification of three independent experiments is presented. Error bars represent the standard deviation. (J) In vitro kinase assay was performed with C. e PLK-1 and the GST-LMN-1 [H] WT or mutated fragments as substrates. The samples were subjected to SDS-PAGE and the gel was revealed using tryptophan fluorescence (Stain-Free; Bio-Rad).

Figure 1—source data 1

Quantification of the in vitro kinase assays.

https://cdn.elifesciences.org/articles/59510/elife-59510-fig1-data1-v1.xlsx
Figure 1—figure supplement 1
Download asset Open asset
PLK-1 but not Aurora A or Cyclin B/Cdk1 phosphorylates LMN-1[H] in vitro.

(A) Context of the study: schematics of the first mitotic division of wild-type and plk-1(-) embryos. In the wild type after fertilization, the female (pink) and the male (blue) pronuclei, which are surrounded by a nuclear envelope, meet at the posterior pole of the embryo. After rotation and centration of the nucleo-centrosomal complex, the nuclear envelope breaks down in the vicinity of the centrosomes and between the juxtaposed pronuclei. This allows the capture of the chromosomes by the microtubules and the merging of the parental chromosomes into a single nucleus. In plk-1(-) embryos, the nuclear envelope persists and physically separates the parental genomes during DNA segregation resulting in the formation of paired nuclei in each blastomere at the two-cell stage embryo. (B) In vitro kinase assays using PLK-1, Cyclin B-Cdk1 or Aurora A kinases and GST-LMN-1 [H] (top panel) or Bora1-224 fragments (bottom panel) as substrates. The samples were subjected to Phos-Tag SDS-PAGE followed by a Coomassie blue staining.

Figure 2 with 1 supplement
Download asset Open asset
PLK-1 interacts with lamins from several species via self and non-self-priming and binding mechanisms.

(A) Schematics of LMN-1 and multiple protein alignments of the head (violet) and the tail (blue) domains of Ce LMN-1 (surrounded in red, bottom lane) with lamins from vertebrates (light green), Drosophila melanogaster (light blue) and other nematodes (light red). Serine residues are highlighted in yellow, Threonine in green and Proline in red. The sequence correspondence and accession numbers are provided in the Supplementary file 3. The black and blue arrows point the C. e LMN-1 residues phosphorylated by PLK-1 in red at the bottom of the alignment. The blue arrows point residues previously shown to be phosphorylated in the C. elegans germline. Putative polo-docking sites are underlined in light blue. (B) (a) Schematics and sequence of the GST-LMN-1 [H] fragment WT or with the indicated substitution of serine and threonine residues by non-phosphorylatable alanine (3A, 4A, and 7A). Polo-docking sites are underlined in light blue. (b) In vitro kinase assay was performed with PLK-1 and the GST-LMN-1 [H] fragments as substrate. The samples were subjected to SDS-PAGE, followed by a Far-Western ligand-binding assay using the Polo-box domain fused to GST (+PBD, upper panel). The bottom panel shows the Stain-Free Blot (Chemidoc, Bio-Rad) of the same membrane. The full experiment is presented in Figure 2—figure supplement 1B. (C) (a) Schematics and sequence of Drosophila melanogaster lamin Dm0 head domain WT or 4A fused to GST. The sites phosphorylated by Cyclin-Cdk1 are circled (Mehsen et al., 2018). These four sites are substituted by alanine in the 4A construct. The canonical polo-docking sites are underlined in light blue. (b) In vitro kinase assay was performed with Cyclin-Cdk1 and the GST-lamin Dm0 [H] fragments as substrate. The samples were subjected to SDS-PAGE, followed by a Far-Western ligand-binding assay using the Polo-box domain fused to GST (+GST-PBD, upper panel). The bottom panel shows the Stain-Free Blot (Chemidoc, Bio-Rad) of the same membrane. The full experiment is presented in Figure 2—figure supplement 1C. (D) (a) Schematics and sequence of human lamin A/C head domain. This fragment contains three S/T-P sites phosphorylatable by Cyclin-Cdk1 kinase including one polo-docking site (underlined in light blue). This specific site is substituted by alanine in the 1A version. (b) In vitro kinase assay was performed with cyclin-cdk1 and the GST-lamin A/C [H] WT or 1A fragments as substrate. The samples were subjected to SDS-PAGE, followed by a Far-Western ligand-binding assay using the Polo-box domain fused to GST (+GST-PBD, upper panel). The bottom panel shows the Stain-Free Blot (Chemidoc, Bio-Rad) of the same membrane. The full experiment is presented in Figure 2—figure supplement 1D.

Figure 2—figure supplement 1
Download asset Open asset
Lamins from several species interact with the Plk1 PBD in a phospho-dependent manner.

(A) In vitro kinase assay was performed with C.e PLK-1 and the GST-LMN-1 head [H] or tail [T] fragments as substrates. The samples were subjected to SDS-PAGE, followed by a Far-Western ligand-binding assay using GST-PBD wild type (a) or the corresponding phosphate pincer (GST-PBD H538A/K540M) mutant (b). The bottom panel shows the Stain-Free Blot (Chemidoc, Bio-Rad) of the same membrane (c, d). GST is present as an impurity from the production of GST-LMN-1 fragments. (B) Full scans of Western blots corresponding to Figure 2B (panels b and d) and including the Far-Western blot using the PBD mutated on the phosphate pincer as control (panels a and c). In vitro kinase assay was performed with PLK-1 and the GST-LMN-1 [H] WT and mutated fragments as substrate. The samples were subjected to SDS-PAGE, followed by a Far-Western ligand-binding assay using GST-PBD wild type (a) or the corresponding phosphate pincer (GST-PBD H538A/K540M) mutant (b). The bottom panel shows the Stain-Free Blot (Chemidoc, Bio-Rad) of the same membrane (c, d). GST is present as an impurity from the production of GST-LMN-1 fragments. (C) Full scans of Western blots corresponding to Figure 2C (panels b and d) and including the Far-Western blot using the PBD mutated on the phosphate pincer as control (panels a and c). In vitro kinase assay was performed with Cyclin-Cdk1 and the GST-lamin Dm0 [H] fragments as substrate. The samples were subjected to SDS-PAGE, followed by a Far-Western ligand-binding assay using GST-PBD wild-type (a) or the corresponding phosphate pincer (GST-PBD H538A/K540M) mutant (b). The bottom panel shows the Stain-Free Blot (Chemidoc, Bio-Rad) of the same membrane (c, d). (D) Full scans of Western blots corresponding to Figure 2D. In vitro kinase assay was performed with Cyclin-Cdk1 and the GST-lamin A/C [H] WT or 1A fragments as substrate. The samples were subjected to SDS-PAGE, followed by a Far-Western ligand-binding assay using GST-PBD wild type (a) or the corresponding phosphate pincer (GST-PBD H538A/K540M) mutant (b). The bottom panel shows the Stain-Free Blot (Chemidoc, Bio-Rad) of the same membrane (c, d).

Figure 3 with 5 supplements
Download asset Open asset
LMN-1-mediated PLK-1 phosphorylation promotes lamina disassembly during mitosis.

(A) Schematics of the approach used to monitor and quantify lamina disassembly during mitosis using spinning disk confocal microscopy in embryos expressing GFP::LMN1 WT or 8A (green) and mCherry-HIS-11 (red). GFP::LMN-1 intensity was quantified over time in the boxed region relative to anaphase onset (time 0). (B) Schematics of GFP::LMN-1 wild-type and 8A with the position of the serine (boxed in yellow) and threonine (boxed in green) residues phosphorylated by PLK-1 in vitro. The eight serine residues substituted by non-phosphorylatable alanine in the head and the tail domains of GFP::LMN-1 8A are S21, S24, S22, S32, T397, T398, S403 and S405. The polo-docking sites are underlined in light blue. (C) Spinning disk confocal micrographs of lmn-1∆ mutant embryos expressing wild-type GFP::LMN-1 (shown alone, and in green in the merged images) and mCherry::HIS-11 (red, in the merged image) exposed to control or plk-1(RNAi). Timings in second are relative to anaphase onset. All panels are at the same magnification. Scale Bar, 10 μm. D- Quantification of GFP::LMN-1 signal intensity above background at the NE in embryos of the indicated genotype during mitosis. The mean +/- SEM is presented for n = 6 embryos for control and n = 5 plk-1(RNAi) embryos. Data were collected from three independent experiments. (E) Spinning disk confocal micrographs of lmn-1∆ mutant embryos expressing GFP::LMN-1 8A (shown alone, and in green in the merged images) and mCherry::HIS-11 (red, in the merged image) exposed to control or plk-1(RNAi). Timings, in seconds, are relative to anaphase onset. All panels are at the same magnification. Scale Bar, 10 μm. (F) Quantification of GFP::LMN-1 8A signal intensity above background at the NE in embryos of the indicated genotype during mitosis. The mean +/- SEM is presented for n = 7 embryos for control and n = 9 for plk-1(RNAi). Data were collected from three independent experiments.

Figure 3—source data 1

Quantification of GFP::LMN-1 WT and 8A signal intensity during mitosis in control and plk-1(RNAi) embryos (related to Figure 3D and F).

https://cdn.elifesciences.org/articles/59510/elife-59510-fig3-data1-v1.xlsx
Figure 3—figure supplement 1
Download asset Open asset
LMN-1 Serine 32 is phosphorylated in a PLK-1-dependent manner in one-cell C. elegans embryos.

Immunostaining of ctrl(RNAi) and plk-1(RNAi) one-cell embryos using phosphospecific [pS32] antibody (shown alone and in red) and GFP::LMN-1 (green). The asterisk denotes non-specific stainings at the centrosomes. Two embryos are presented.

Figure 3—video 1
Download asset
Lamina disassembly in early embryos expressing GFP::LMN-1 and mCherry::HIS-11 exposed to control (video 1) or plk-1(RNAi) (video 2).
Figure 3—video 2
Download asset
Lamina disassembly in early embryos expressing GFP::LMN-1 and mCherry::HIS-11 exposed to control (video 1) or plk-1(RNAi) (video 2).
Figure 3—video 3
Download asset
Lamina disassembly in early embryos expressing GFP::LMN-1 8A and mCherry::HIS-11 exposed to control (video 3) or plk-1(RNAi) (video 4).
Figure 3—video 4
Download asset
Lamina disassembly in early embryos expressing GFP::LMN-1 8A and mCherry::HIS-11 exposed to control (video 3) or plk-1(RNAi) (video 4).
Figure 4
Download asset Open asset
Non-phosphorylatable LMN-1 8A is sufficient to cause the appearance of embryos with a paired nuclei phenotype.

(A) Differential interference contrast micrographs and corresponding schematics of the first two divisions in wild type (WT) (a–e) and lmn-1 8A (f–j) embryos at 23°C. Black arrowheads highlight paired nuclei in lmn-1 8A mutant embryos. PNM: Pronuclear meeting, NEBD: nuclear envelope breakdown. Scale Bar, 10 μm. (B) Percentage of WT and lmn-1 8A two-cell stage embryos presenting 0 (green bars), 1 (orange bars) or 2 (red bars) paired nuclei at the two-cell stage at 23°C. The number of embryos analyzed (n) is indicated on the right and was generated by aggregation over more than three independent experiments. The values inside the bars represent the percentage of embryos of a given phenotype. (C) Graph presenting the embryonic viability (%) determined from wild-type (n = 21) and lmn-1 8A (n = 27) mutant animals. Data were collected from two independent experiments containing each two replicates. The results are presented as means ± SEM. **** indicates p<0.0001. (D–E) Confocal images of fixed wild-type and lmn-1 8A mutant one-cell embryos in pro-metaphase, metaphase, anaphase and telophase, stained with LMN-1 antibodies (green) and counterstained with DAPI (magenta). All panels are at same magnification. Scale Bar, 10 μm.

Figure 4—source data 1

Progeny test analysis of WT versus lmn-1 8A mutants (related to Figure 4C).

https://cdn.elifesciences.org/articles/59510/elife-59510-fig4-data1-v1.xlsx
Figure 5 with 1 supplement
Download asset Open asset
PLK-1 localizes at sites of lamina depolymerization in vivo and disassembles C.e lamin filaments in vitro.

(A) Images from a time-lapse spinning disk confocal movie showing the spatiotemporal localization of PLK-1::sGFP to centrosomes, chromosomes and to the NE during the first mitosis of the C. elegans zygote. Orange arrows point to the regions of the NE located in the regions of the pronuclei where PLK-1::sGFP is excluded before NEBD. NEBD is defined as the time point at which the nuclear envelope starts to deform (white arrows). Yellow arrows point to the region of the NE surrounding the mitotic spindle where PLK-1::sGFP accumulates. Times are in minutes:seconds relative to anaphase onset. Scale bar, 5 μm. (B) Flow-chart of the approach used to purify LMN-1 from E. coli and to assemble LMN-1 filaments in vitro. Coomassie blue staining of purified 6xHis-LMN-1 before and after cleavage of the 6xHis tag with TEV protease. As the TEV protease is also tagged with the 6xHis tag (not mentioned in the figure), the protease is removed from the LMN-1 preparation after purification on Ni2+ column (step 2). (C) Negative staining electron microscopy micrograph of C. elegans lamin filaments assembled from purified LMN-1. Scale Bar, 100 nm. D- In vitro kinase assay was performed with Mg/ATP alone as control (a) or with PLK-1 plus Mg/ATP (b) and LMN-1 filaments as substrate. The samples were taken at the indicated time, centrifuged and the pellet (P) and supernatant (S) fractions were subjected to SDS-PAGE and the gel was stained with Coomassie brilliant blue.

Figure 5—figure supplement 1
Download asset Open asset
PLK-1 kinase promotes LMN-1 filaments disassembly in vitro.

Negative staining electron microscopy micrograph of C. elegans lamin filaments assembled from purified LMN-1 after 40 min incubation with Mg/ATP as control (A, C) or Mg/ATP and PLK-1 kinase (B, D). Scale Bar, 200 nm.

Figure 6 with 3 supplements
Download asset Open asset
Paternally- and maternally-derived chromatin remain physically separated during mitosis in lmn-1 8A mutant one-cell embryos.

(A) Schematics of the live-imaging approach used to differentially label parental chromosomes. The female pronuclei of the Dendra2-H2B-expressing strain are photoconverted (405 nm laser beam) in the early zygote changing the fluorescence of the female pronucleus from green-to-red and the embryos are imaged until the 2 cell stage. (B) Representative spinning disk confocal images are taken from different wild-type (a, d) and lmn-1 8A (e, f) mutant embryos. Shown are maximum projections of image stacks. Nuclei are in mitotic prophase (a, e), metaphase (b, f) anaphase (c, g) and telophase (d, h). Scale Bar, 10 μm. (C-D) Spinning disk confocal micrographs of WT and lmn-1 8A mutant one-cell stage embryos expressing the INM protein LEM-2 fused to GFP (GFP::LEM-2, shown alone on the right of the panels and in green in the merged images) and mCherry::HIS-58 (magenta). In the control embryo (left), the nuclear envelope breaks as the chromosomes align at the metaphase plate (orange arrows), allowing the chromosomes from the sperm and oocyte pronuclei to merge on the spindle before their segregation. In the lmn-1 8A mutant embryos, the nuclear envelope remains intact and acts as a physical barrier that separates the chromosomes from the two pronuclei during their segregation. On the bottom panels, schematics illustrate the presence of a membrane gap in wild-type as opposed to lmn-1 8A embryos. Scale Bar, 10 μm. Times are in seconds relative to anaphase onset.

Figure 6—figure supplement 1
Download asset Open asset
lmn-1 8A mutant one-cell embryos are defective in membrane gap formation but properly align parental chromosomes.

(A) Spinning disk confocal micrographs of representative early lmn-1 8A mutant embryos expressing GFP::LEM-2 (green) and mCherry::HIS-58 (magenta) in metaphase, anaphase and telophase. 7/12 early embryos properly aligned chromosomes on the metaphase plate. Among these seven embryos, five presented a double paired-nuclei phenotype and two did not present paired nuclei. Scale Bar, 10 μm. (B) Percentage of lmn-1 8A early embryos with aligned (pink) or misaligned chromosomes (brown).

Figure 6—video 1
Download asset
Control (video 1) or lmn-1 8A (video 2) mutant embryos expressing GFP::LEM-2 and mCherry::HIS-58.
Figure 6—video 2
Download asset
Control (video 1) or lmn-1 8A (video 2) mutant embryos expressing GFP::LEM-2 and mCherry::HIS-58.
Figure 7
Download asset Open asset
PLK-1 dynamics at the nuclear envelope dictates the timing of lamina depolymerization during mitosis The maternal and paternal chromosomes are colored in pink and blue respectively.

Polo-like kinase1 with the kinase domain (KD) in dark blue and the polo-box domain (PBD) in orange. Phosphorylated residues (P) circled in dark blue are modified by PLK-1.

Tables

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Strain, strain background (Caenorhabditis elegans)C. elegans N2 BristolCGChttp://www.cgc.cbs.umn.edu/strain.php?id=10570
Strain, strain background (C. elegans)[gfp::lmn-1] MosSCI: lmn-1(tm1502) I; jfSi68[Plmn-1::gfp cb-unc-119(+)] IILink et al., 2018, #49005UV120
Strain, strain background (C. elegans)[gfp::lmn-1S8A] MosSCI: lmn-1(tm1502) I; jfSi89[Plmn-1S(21,22,24,32,397,398,403,405)A::gfp cb-unc-119(+)] IILink et al., 2018, #49005UV122
Strain, strain background (C. elegans)lmn-1(jf140[S21,22,24,32,397,398, 403,405A]) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III)This studyUV2059V. Jantsch Lab
Strain, strain background (C. elegans)tonSi1[mex-5p::Dendra2::his-66::tbb-2 3’UTR + Cbr-unc-119(+)]IIBolková and Lanctôt, 2016, #90027JBL1
Strain, strain background (C. elegans)lmn-1(jf140[S21,22,24,32,397,398, 403,405A]) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III);tonSi1[mex-5p::Dendra2::his-66::tbb-2 3’UTR + Cbr-unc-119(+)]IIThis studyWLP831L. Pintard Lab
Strain, strain background (C. elegans)Itls37 [(pAA64) pie-1p::mCherry::his-58 + unc-119(+)]IV qals3507 [pie-1::GFP::LEM-2 + unc-119(+)]CGCOD83
Strain, strain background (C. elegans)lmn-1S8AS(21,22,24,32,397,398,403, 405)A Itls37 [(pAA64) pie-1p::mCherry::his-58 + unc-119(+)]IV qals3507 [pie-1::GFP::LEM-2 + unc-119(+)]This studyWLP833L. Pintard Lab
Strain, strain background (C. elegans)plk-1(lt17) ([plk-1::sgfp] loxp)IIIMartino et al., 2017, #66043OD2425
Strain, strain background (Escherichia coli)BL21(DE3)Sigma-AldrichCMC0016
AntibodyLMN-1
(Rabbit polyclonal)		Novus BiologicalsCat#38530002
RRID:AB_10005072		(1:100 IF)
(1:1,000 WB)
AntibodyLMN-1Ser32pi
(Rabbit polyclonal)		Link et al., 2018, #49005V. Jantsch Lab
(Home made)		(1:10 IF)
AntibodyPlk1 (human)
(Mouse monoclonal)		Merck MilliporeCat#05–844
RRID:AB_310836		(1:1,000 WB)
AntibodyGST
(Rabbit polyclonal)		This studyL. Pintard Lab
(Home made)		(1:1,000 WB)
AntibodyAnti-Mouse IgG (Fab specific) -Peroxidase antibody
(produced in goat)		SigmaCat#A9917
RRID:AB_258476		(1:5,000 WB)
AntibodyAnti-Rabbit IgG (whole molecule) -Peroxidase antibody
(Produced in goat)
SigmaCat#A0545
RRID:AB_257896		(1:3,000 WB)
AntibodyAnti-Rabbit IgG (H+L) Cross-Adsorbed Secondary
Antibody, Alexa Fluor 568 (Produced in goat)
Fluor 568		InvitrogenCat#A-11011
RRID:AB_143157		(1:1,000 IF)
AntibodyAnti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Produced in goat)
InvitrogenCat#A-11001
RRID:AB_2534069		(1:1,000 IF)
Chemical compound, drugCoomassie R250SigmaCat#B014925G
Chemical compound, drugPonceau RedSigmaCat#A1405
Chemical compound, drugVECTASHIELD Mounting Medium with DAPIEurobioCat#H-1200
Chemical compound, drugIPTGEuromedexCat#EU0008-B
Chemical compound, drugAdenosine TriPhosphate (ATP)SigmaCat#A2383
Chemical compound, drugγ-[32P] Adenosine TriPhosphate (ATP)500 μCi 3000 Ci/mmolPerkinElmerCat#NEG002A500U
Chemical compound, drugPhos-TagAcrylamide AAL-107WAKO-SOBIODACat#W1W304-93521
Chemical compound, drugImidazoleSigmaCat#I202
Chemical compound, drugGlutathioneSigmaCat#G4251
Chemical compound, drugHiTrap Chelating HP 5 × 1 mLGE HealthcareCat#17-0408-01
Chemical compound, drugGlutathion Sepharose 4B Fast FlowGE HealthcareCat#17-0756-01
Chemical compound, drugPfuPromegaCat#M7741
Chemical compound, drugDpnIBiolabsCat#R0176S
Commercial assay or kitECL reagentMilliporeCat#WBKLS0500
Commercial assay or kitBP Clonase II Enzyme Mix (Gateway cloning)InvitrogenCat#11789–020
Commercial assay or kitLR Clonase II Enzyme Mix (Gateway cloning)InvitrogenCat#11791–020
Recombinant proteinHuman Cyclin B-Cdk1 kinaseNew England BiolabsCat#P6020L
Recombinant DNA reagentL4440 (RNAi Feeding vector)Kamath et al., 2001, #2201N/A
Recombinant DNA reagentplk-1 cloned into L4440Kamath et al., 2003, #46063Arhinger Library
Recombinant DNA reagentpDESTttTi5605[R4-R3] for MOS insertion on Chromosome IIFrøkjaer-Jensen et al., 2008, #79702pCFJ150
Addgene plasmid # 19329
Recombinant DNA reagentMOS transposase Pglh-2::MosTase::glh-2utrFrøkjaer-Jensen et al., 2008, #79702pJL43.1
Addgene plasmid # 19332
Recombinant DNA reagentPrab-3::mCherryFrøkjaer-Jensen et al., 2008, #79702pGH8
Recombinant DNA reagentPmyo-2::mCherry::unc-54Frøkjaer-Jensen et al., 2008, #79702pCFJ90
Recombinant DNA reagentEntry clone for Gateway pDONR221 (lmn-1 CDS was cloned in this vector)Multistite Gateway Kit, Thermo Fischer ScientificCat# 12537–023
Recombinant DNA reagentpDONR P4-P1R (the lmn-1 5’ UTR with and without GFP was cloned in this vector)Multistite Gateway Kit, Thermo Fischer ScientificCat# 12537–023
Recombinant DNA reagentpDONRTM P2r-P3 (thelmn-1 3’UTR was cloned in this vector)Multistite Gateway Kit, Thermo Fischer ScientificCat# 12537–023
Recombinant DNA reagentMinigene from IDT for generating the lmn-18A containing the whole CDS of lmn-1(S21,22,24,32,397, 398,403,405A)Link et al., 2018, #49005N/A
Recombinant DNA reagentPlmn-1_gfp::lmn-1_lmn-1 3’UTR in pCFJ150Link et al., 2018, #49005N/A
Recombinant DNA reagentPlmn-1_gfp::lmn-1(S8A) lmn-1 3’UTR in pCFJ150Link et al., 2018, #49005N/A
Recombinant DNA reagentGal4 pDEST DBNoatynska et al., 2010, #23221pMG97
Recombinant DNA reagentGal4 pDEST DB-PLK-1 PBDNoatynska et al., 2010, #23221pMG477
Recombinant DNA reagentGal4 pDEST DB-PLK-1 PBD H542A, K544MNoatynska et al., 2010, #23221pMG538
Recombinant DNA reagentpGEX-4T (GST)GE HealthcareCat#GE28-9545-49
Recombinant DNA reagentpFasTBAC Hta PLK-1 C. elegansTavernier et al., 2015, #22388pLP871
Recombinant DNA reagentpGEX-6p1 GST-PLK-1 PBD H. sGift I. SumaraN/A
Recombinant DNA reagentpGEX-6p1 GST-PLK-1 PBD H538A/K540M H. sGift I. SumaraN/A
Recombinant DNA reagentpDONR201 TEV-Aurora A H. sThis studypLP1884
Recombinant DNA reagentHis-GST-TEV-Aurora A H. sThis studypLP2067
Recombinant DNA reagentpBABE-puro-GFP-wt-lamin AGift B. CisnerosAddgene #17662
Recombinant DNA reagentcDNA LMN-1 13A (S18A, S21A, S22A, S24A, S32A, S35A, T36A, T390A, T397A, S398A, S403A, S405A, S406A)
Generate by Thermofisher		ThermoFisherN/A
Recombinant DNA reagentpDONR201ThermoFisherN/A
Recombinant DNA reagentpDONR201 LMN-1 [H] (aa1-47)This studypLP2234
Recombinant DNA reagentpDONR201 LMN-1 [H] (aa1-47) 3A S18A S35A T36AThis studypLP2277
Recombinant DNA reagentpDONR201 LMN-1 [H] (aa1-47) 4A S21A S22A S24A S32AThis studypLP2236
Recombinant DNA reagentpDONR201 LMN-1 [H] (aa1-47) 7A S18A S21A S22A S24A S32A S35A T36AThis studypLP2276
Recombinant DNA reagentpDONR201 LMN-1 [T] (aa387-548)This studypLP2270
Recombinant DNA reagentpDONR201 LMN-1
[T1] (aa387-436)		This studypLP2249
Recombinant DNA reagentpDONR201 LMN-1 [T1] 4A (aa387-436) T397A S398A S403A S405AThis studypLP2250
Recombinant DNA reagentpDonR201 LMN-1 [T1] 6A (aa387-436) T390A, T397A, S398A, S403A, S405A, S406AThis studypLP2269
Recombinant DNA reagentpDONR201 cDNA of lamin Dm0 from Drosophila melanogasterMehsen et al., 2018, #44350N/A
Recombinant DNA reagentpDONR201 cDNA of lamin with 7 mutations of the CDK motifs from Drosophila melanogaster
T12A, T20A, S42A, S45A, T413A, T435A, T440A		Mehsen et al., 2018, #44350N/A
Recombinant DNA reagentpDONR201 Lamin Dm0 [H] (aa1-57)This studypLP2314
Recombinant DNA reagentpDONR201 Lamin Dm0 [H] (aa1-57) 4A T12A T20A S42A S44AThis studypLP2315
Recombinant DNA reagentpDONR201 Lamin A/C [H] (aa1-34)This studypLP2316
Recombinant DNA reagentpDONR201 TEV-Bora 1–224 HsThis studypLP1848
Recombinant DNA reagentpDEST17 His-TEV-Bora 1–224 HsThis studypLP1850
Recombinant DNA reagentpDEST15ThermoFisherCat#11802014
Recombinant DNA reagentpDEST15 GST- LMN-1 [H] (aa 1–47)This studypLP2235
Recombinant DNA reagentpDEST15 GST- LMN-1 [H] (aa 1–47) 3A S18A S35A T36AThis studypLP2282
Recombinant DNA reagentpDEST15 GST- LMN-1 [H] (aa 1–47) 4A S21A S22A S24A S32AThis studypLP2237
Recombinant DNA reagentpDEST15 GST- LMN-1 [H] (aa1-47) 7A S18A S21A S22A S24A S32A S35A T36AThis studypLP2281
Recombinant DNA reagentpDEST15 GST- LMN-1 [H] (aa1-47) 9A S18A S21A S22A S24A S32A S35A T36A T40A S41AThis studypLP2393
Recombinant DNA reagentpDEST15 GST- LMN-1 [T] (aa387-548)This studypLP2271
Recombinant DNA reagentpDEST15 GST- LMN-1 [T1] (aa387-436)This studypLP2253
Recombinant DNA reagentpDEST15 GST- LMN-1 [T1] 4A (aa387-436) T397A S398A S403A S405AThis studypLP2254
Recombinant DNA reagentpDEST15 GST- LMN-1 [T1] 6A (aa387-436) T390A, T397A, S398A, S403A, S405A, S406AThis studypLP2298
Recombinant DNA reagentpDEST15 GST- Lamin Dm0 [H] (aa1-57)This studypLP2322
Recombinant DNA reagentpDEST15 GST- Lamin Dm0 [H] (aa1-57) 4A T12A T20A S42A S44AThis studypLP2323
Recombinant DNA reagentpDEST15 GST- Lamin A/C [H] (aa1-34)This studypLP2324
Recombinant DNA reagentpDEST15 GST- Lamin A/C [H] (aa1-34)1A T18AThis studypLP2362
Recombinant DNA reagentpET28bNovagenCat#69864
Recombinant DNA reagentpET28b (His linker-TEV-linker Nter) LMN-1This studypLP2231
Recombinant DNA reagentAlt-R CRISPR-Cas9 tracrRNA, 100 nmolIDTCat#1072534
Sequence-based reagentPrimers for cloning and site-directed mutagenesis (see oligonucleotide sequences table S4 source)This studyN/A
Software, algorithmClustal OmegaEMBL-EBIhttps://www.ebi.ac.uk/Tools/msa/clustalo/
Software, algorithmJalviewWaterhouse et al., 2009, #45561https://www.jalview.org/
Software, algorithmAdobe Illustrator CS6Adobehttps://www.adobe.com/products/illustrator.html
Software, algorithmAdobe Photoshop CS4Adobehttps://www.adobe.com/products/photoshop.html
Software, algorithmImage JNIH;
Schneider et al., 2012, #74926		https://imagej.nih.gov/ij/
Software, algorithmZENZeisshttps://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
Software, algorithmPRISMGraphpadhttps://www.graphpad.com/
Software, algorithmMetamorphMolecular Deviceshttps://www.metamorph.com/
Software, algorithmProteome Discoverer 2.2Thermo Scientifichttps://www.thermofisher.com/store/products/OPTON-30945#/OPTON-30945
Software, algorithmMatrix Science 5.1Mascot Serverhttps://www.matrixscience.com/

Additional files

Supplementary file 1

LC-MS/MS analysis of phosphorylated LMN-1 in vitro.

https://cdn.elifesciences.org/articles/59510/elife-59510-supp1-v1.xlsx
Supplementary file 2

Sequence analysis of LMN-1 phospho-sites.

Phosphorylated serine and threonine residues (pS and pT) are highlighted in yellow and green respectively. Most phosphosites match the previously described Plk1 consensus motifs [L(Φ)-D/E/N/Q-X-pS/pT-L(Φ) or p[S/T]-F] (Santamaria et al., 2011; Kettenbach et al., 2011). D/E/N/Q residues are highlighted in blue, hydrophobic (Φ) in orange and F. in red.

https://cdn.elifesciences.org/articles/59510/elife-59510-supp2-v1.xlsx
Supplementary file 3

Accession numbers of lamin protein sequences.

https://cdn.elifesciences.org/articles/59510/elife-59510-supp3-v1.docx
Supplementary file 4

Oligonucleotides used in this study.

https://cdn.elifesciences.org/articles/59510/elife-59510-supp4-v1.docx
Transparent reporting form
https://cdn.elifesciences.org/articles/59510/elife-59510-transrepform-v1.docx

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Griselda Velez-Aguilera
  2. Sylvia Nkombo Nkoula
  3. Batool Ossareh-Nazari
  4. Jana Link
  5. Dimitra Paouneskou
  6. Lucie Van Hove
  7. Nicolas Joly
  8. Nicolas Tavernier
  9. Jean-Marc Verbavatz
  10. Verena Jantsch
  11. Lionel Pintard
(2020)
PLK-1 promotes the merger of the parental genome into a single nucleus by triggering lamina disassembly
eLife 9:e59510.
https://doi.org/10.7554/eLife.59510