Dendritic coincidence detection is thought fundamental to neuronal processing yet remains largely unexplored in awake animals. Specifically, the underlying dendritic voltage-calcium relationship has not been directly addressed. Here, using simultaneous voltage and calcium two-photon imaging of Purkinje neuron spiny dendrites, we show how coincident synaptic inputs and resulting dendritic spikes modulate dendritic calcium signaling during sensory stimulation in awake mice. Sensory stimulation increased the rate of post-synaptic potentials and dendritic calcium spikes evoked by climbing fiber and parallel fiber synaptic input. These inputs are integrated in a time-dependent and non-linear fashion to enhance the sensory evoked dendritic calcium signal. Intrinsic supralinear dendritic mechanisms, including voltage-gated calcium channels and metabotropic glutamate receptors, are recruited cooperatively to expand the dynamic range of sensory evoked dendritic calcium signals. This establishes how dendrites can use multiple interplaying mechanisms to perform coincidence detection, as a fundamental and ongoing feature of dendritic integration in behaving animals.
Matlab codes are available at: https://github.com/cjroome/Roome_and_Kuhn_2020Data is available at: https://doi.org/10.5061/dryad.6hdr7sqzt
Dendritic coincidence detection in Purkinje neurons of awake miceDryad Digital Repository, doi:10.5061/dryad.6hdr7sqzt.
- Christopher J Roome
- Bernd Kuhn
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Animal experimentation: All animal procedures were conducted in accordance with guidelines of the Okinawa Institute of Science and Technology Institutional Animal Care and Use Committee in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC International)-accredited facility, under protocol numbers: 2016-170, 2019-279.
- Megan R Carey, Champalimaud Foundation, Portugal
© 2020, Roome & Kuhn
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