Dendritic coincidence detection in Purkinje neurons of awake mice

Abstract
Data availability
Article and author information
Metrics

Abstract

Dendritic coincidence detection is thought fundamental to neuronal processing yet remains largely unexplored in awake animals. Specifically, the underlying dendritic voltage-calcium relationship has not been directly addressed. Here, using simultaneous voltage and calcium two-photon imaging of Purkinje neuron spiny dendrites, we show how coincident synaptic inputs and resulting dendritic spikes modulate dendritic calcium signaling during sensory stimulation in awake mice. Sensory stimulation increased the rate of post-synaptic potentials and dendritic calcium spikes evoked by climbing fiber and parallel fiber synaptic input. These inputs are integrated in a time-dependent and non-linear fashion to enhance the sensory evoked dendritic calcium signal. Intrinsic supralinear dendritic mechanisms, including voltage-gated calcium channels and metabotropic glutamate receptors, are recruited cooperatively to expand the dynamic range of sensory evoked dendritic calcium signals. This establishes how dendrites can use multiple interplaying mechanisms to perform coincidence detection, as a fundamental and ongoing feature of dendritic integration in behaving animals.

Data availability

Matlab codes are available at: https://github.com/cjroome/Roome_and_Kuhn_2020Data is available at: https://doi.org/10.5061/dryad.6hdr7sqzt

The following data sets were generated

1. Roome CJ
2. Kuhn B
(2020) Dendritic coincidence detection in Purkinje neurons of awake mice
Dryad Digital Repository, doi:10.5061/dryad.6hdr7sqzt.

https://doi.org/10.5061/dryad.6hdr7sqzt

Article and author information

Author details

Christopher J Roome

Optical Neuroimaging, OIST Graduate University, Onna, Japan

For correspondence
christopher.roome@oist.jp

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-8936-668X
Bernd Kuhn

Optical Neuroimaging, OIST Graduate University, Onna, Japan

For correspondence
bkuhn@oist.jp

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-6852-2433

Funding

Okinawa Institute of Science and Technology Graduate University

Christopher J Roome
Bernd Kuhn

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: All animal procedures were conducted in accordance with guidelines of the Okinawa Institute of Science and Technology Institutional Animal Care and Use Committee in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC International)-accredited facility, under protocol numbers: 2016-170, 2019-279.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.