Single nuclei RNA-seq of mouse placental labyrinth development

Abstract
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Abstract

The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell types of this organ difficult. Here, we address questions concerning the ontogeny, specification, and function of the cell types of a representative hemochorial placenta by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic development focusing on the exchange interface, the labyrinth. Timepoints extended from progenitor driven expansion through terminal differentiation. Analysis by snRNA-seq identified transcript profiles and inferred functions, cell trajectories, signaling interactions, and transcriptional drivers of all but the most highly polyploid cell types of the placenta. These data profile placental development at an unprecedented resolution, provide insights into differentiation and function across time, and provide a resource for future study.

Data availability

Sequencing data have been deposited in GEO under accession code GSE152248.

The following data sets were generated

1. Marsh BP
2. Blelloch RH
(2020) Single nuclei RNA-seq of mouse placental labyrinth development
NCBI Gene Expression Omnibus, GSE152248.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE152248

Article and author information

Author details

Bryan Marsh

Department of Urology, University of California, San Francisco, San Francisco, United States

Competing interests
The authors declare that no competing interests exist.
Robert Blelloch

Department of Urology, University of California, San Francisco, San Francisco, United States

For correspondence
robert.blelloch@ucsf.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-1975-0798

Funding

Eunice Kennedy Shriver National Institute of Child Health and Human Development (P50 HD055764)

Robert Blelloch

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) (Approval number: AN173513) of the University of California - San Francisco.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.