Generation of stress fibers through myosin-driven re-organization of the actin cortex
- University of Helsinki, Finland
Abstract
Contractile actomyosin bundles, stress fibers, govern key cellular processes including migration, adhesion, and mechanosensing. Stress fibers are thus critical for developmental morphogenesis. The most prominent actomyosin bundles, ventral stress fibers, generated through coalescence of pre-existing stress fiber precursors. However, whether stress fibers can assemble through other mechanisms has remained elusive. We report that stress fibers can also form without requirement of pre-existing actomyosin bundles. These structures, which we named cortical stress fibers, are embedded in the cell cortex and assemble preferentially underneath the nucleus. In this process, non-muscle myosin II pulses orchestrate the reorganization of cortical actin meshwork into regular bundles, which promote reinforcement of nascent focal adhesions, and subsequent stabilization of the cortical stress fibers. These results identify a new mechanism by which stress fibers can be generated de novo from the actin cortex, and establish role for stochastic myosin pulses in the assembly of functional actomyosin bundles.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files for pulse quantification are provided in GitHub (https://github.com/UH-LMU/lmu-users/tree/master/jaakko/NMIIA_pulses).
Article and author information
Author details
Funding
Sigrid Juselius Foundation (4708344)
- Pekka Lappalainen
Aatos Erkko Foundation (4704407)
- Pekka Lappalainen
Academy of Finland (294174)
- Sari Tojkander
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Reinhard Fässler, Max Planck Institute of Biochemistry, Germany
Version history
- Received: July 3, 2020
- Accepted: January 27, 2021
- Accepted Manuscript published: January 28, 2021 (version 1)
- Accepted Manuscript updated: January 29, 2021 (version 2)
- Version of Record published: February 11, 2021 (version 3)
- Version of Record updated: April 20, 2022 (version 4)
Copyright
© 2021, Lehtimäki et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Generation of stress fibers through myosin-driven re-organization of the actin cortex
eLife 10:e60710.
https://doi.org/10.7554/eLife.60710
Further reading
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- Cell Biology
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