ASIC1a is required for neuronal activation via low-intensity ultrasound stimulation in mouse brain
- National Taiwan University, Taiwan
- National Taiwan University Hospital, Taiwan
- Academia Sinica, Taiwan
Abstract
CNS neurons have no sensory function, protected by the skull. For this reason, brain neuromodulation by ultrasound were either done at a high intensity or through auditory nerves. We demonstrate in this study CNS neurons react to ultrasound stimulation at an intensity (5 mW/cm2) far lower than typical therapeutic ultrasound (>30 mW/cm2). Using micropipette ultrasound in calcium imaging, we show ASIC1a channels play a role in the reactions of CNS neurons to ultrasound, pointing to the molecular basis for direct ultrasound neuromodulation at low intensity. Furthermore, we also show evidence of neurogenesis with the same ultrasound stimulation, suggesting potential therapeutic translation.
Data availability
All data generated relevant to this study are included in the manuscript are presented in the manuscript either as main figures or as supplementary figures. Source data files will be provided when there is a need.
Article and author information
Author details
Funding
Ministry of Science and Technology, Taiwan (MOST 107-2221-E-002-068-MY3)
- Jaw-Lin Wang
Ministry of Science and Technology, Taiwan (MOST108-2321-B-002-047)
- Jaw-Lin Wang
Ministry of Science and Technology, Taiwan (MOST 108-2321-B-002-061-MY2)
- Jaw-Lin Wang
National Health Research Institutes (NHRI-EX109-10924EI)
- Jaw-Lin Wang
Ministry of Science and Technology, Taiwan (MOST 108-2321-B-001-028-MY2)
- Chih-Cheng Chen
Ministry of Science and Technology, Taiwan (MOST 110-2321-B001-010)
- Chih-Cheng Chen
National Taiwan University (NTU-CC-107L891105)
- Jaw-Lin Wang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: The animal work has been performed following the recommendations in the Guide for the Care and Use of Laboratory Animals of National Taiwan University. All of the animals handling complies to the IACUC protocol #20190055 of National Taiwan University Hospital.
Copyright
© 2021, Lim et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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ASIC1a is required for neuronal activation via low-intensity ultrasound stimulation in mouse brain
eLife 10:e61660.
https://doi.org/10.7554/eLife.61660
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