Release probability increases towards distal dendrites boosting high-frequency signal transfer in the rodent hippocampus
- Queen Square UCL Institute of Neurology, University College London, United Kingdom
Figures
Figure 1 with 1 supplement
Optical quantal analysis at individual CA3-CA1 synapses reports higher release probability towards distant dendrites.
(A) CA1 pyramidal cell held in whole-cell mode (acute hippocampal slice), dialysed with 50 µM AF 594 and 300 µM Fluo-8 (75 µm z-stack average, λx2p = 800 nm; AF 594 channel). Dotted rectangles (S1 …
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Figure 1—source data 1
Original data readout for Figure 1C-E and Figure 1—figure supplement 1D.
- https://cdn.elifesciences.org/articles/62588/elife-62588-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
Optical quantal analysis at individual CA3-CA1 synapses in acute hippocampal slices: second-order dendrite.
(A) CA1 pyramidal cell held in whole-cell mode dialysed with 50 µM AF 594 and 400 µM OGB-1 (75 µm z-stack average, λx2p = 800 nm; DIC + AF 594 channel combined). Patch pipette (patch) and …
Figure 2 with 1 supplement
Selected features of excitatory synapses with respect to their dendritic location.
(A) Average release probability (Pr) at individual synapses plotted against distance to the first dendrite branching point. Solid line, linear regression (p value to reject H0 = zero slope and …
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Figure 2—source data 1
Original data readout for Figure 2A-F and Figure 2—figure supplement 1A-B.
- https://cdn.elifesciences.org/articles/62588/elife-62588-fig2-data1-v2.xlsx
Figure 2—figure supplement 1
Selected features of excitatory synapses with respect to their dendritic location.
(A) Average probability of the second release (paired-pulse stimuli 50 ms apart) plotted against distance to the soma; sample mean ± SEM: 0.633 ± 0.20 (n = 41). (B) Paired-pulse ratio (as P2/P1 in C)…
Figure 3 with 1 supplement
Evoked glutamate release from Schaffer collaterals shows lower paired-pulse ratios at greater distances from pyramidal cell bodies.
(A) Experimental design: area of the hippocampal slice with iGluSnFR expressed in neuronal membranes (green channel); arrow, measured distance d between CA1 pyramidal cell body layer and the axonal …
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Figure 3—source data 1
Original data readout for Figure 3C-D and Figure 3—figure supplement 1C.
- https://cdn.elifesciences.org/articles/62588/elife-62588-fig3-data1-v2.xlsx
Figure 3—figure supplement 1
Optical (multi-synaptic) glutamate signal evoked by short bursts of Schaffer collateral stimulation at varied distances from s.pyramidale.
(A) Examples of recorded ROIs (iGluSnFR green fluorescence channel, dotted ovals) in s. radiatum, with groups of stimulation-responding tentative axonal boutons expressing iGluSnFR, at different …
Figure 4 with 1 supplement
Computer simulations of CA1 pyramidal cell with stochastic excitatory synapses.
(A) Diagram, NEURON model of a reconstructed CA1 pyramidal cell (Migliore et al., 1999) (ModelDB 2796; variable time step dt, t = 34°C). 50 excitatory inputs (blue dots) generate bi-exponential …
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Figure 4—source data 1
Original data readout for Figure 4A–D and Figure 4—figure supplement 1A–F.
- https://cdn.elifesciences.org/articles/62588/elife-62588-fig4-data1-v2.xlsx
Figure 4—figure supplement 1
Simulating the experiment-based kinetics of short-term plasticity (STP) in the CA3-CA1 circuit.
(A) Traces show simulated average somatic response to five-burst 20 Hz stimuli (NEURON model as in Figure 4A), with synaptic Pr values distributed either uniformly randomly (black line), or in …
Author response image 1
Author response image 2
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | Picrotoxin | Tocris | Cat. # 1128 | |
| Chemical compound, drug | D-Serine | Sigma Aldrich | Cat. # S4250 | |
| Chemical compound, drug | Oregon Green 488 BAPTA-1, Hexapotassium Salt | ThermoFisher | Cat. #O6806 | |
| Chemical compound, drug | Alexa Fluor-594 Hydrazide | ThermoFisher Scientific | Cat.# A10438 | |
| Chemical compound, drug | Fluo-8, potassium salt | Stratech Scientific | Cat.# 21087-AAT | Original Source: (Teflabs) No longer exists |
| Chemical compound, drug | Fluo-4, Pentapotassium Salt | ThermoFisher Scientific | Cat.# F14200 | |
| Chemical compound, drug | Agarose | Sigma-Aldrich | Cat. #9539; CAS: 9012-36-6 | Freshly prepared (4%) |
| Chemical compound, drug | D-glucose | Sigma-Aldrich | Cat. #G8270; CAS: 50-99-7 | Freshly prepared (10 mM) |
| Chemical compound, drug | KCl | Sigma-Aldrich | Cat. #P9333; CAS: 7447-40-7 | |
| Recombinant DNA reagent | AAV9.hSynap.iGluSnFr.WPRE.SV40 | Penn Vector Core | Addgene Cat. # 98929-AAV9 | |
| Strain, strain background (Sprague-Dawley rat) | Sprague-Dawley | Charles River UK | Crl: CD (SD) Strain: 0204 | |
| Strain, strain background (C57BL/6J mouse) | C57BL/6J | Charles River UK | C57BL/6NCrl Strain: 0159 | |
| Software, algorithm | ImageJ | NIH | RRID:SCR_003070 https://imagej.nih.gov/ij/ | |
| Software, algorithm | pClamp10 | Molecular Devices | RRID:SCR_011323 https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite | |
| Software, algorithm | OriginPro | OriginLab Inc | RRID:SCR_014212 https://www.originlab.com/origin | |
| Software, algorithm | MES 4.x-5.x | Femtonics Ltd. | RRID:SCR_018309 https://uk.mathworks.com/products/connections/product_detail/femtonics-mes.html | |
| Software, algorithm | WinWCP Versions 4.x-5.x | Strathclyde Electrophysiology Software | RRID:SCR_014270 http://spider.science.strath.ac.uk/sipbs/software_ses.htm | |
| Software, algorithm | Matlab | Mathworks | RRID:SCR_001622 https://uk.mathworks.com/products/matlab.html | |
| Software, algorithm | NEURON 7.6 × 64 | https://www.neuron.yale.edu/neuron/ | RRID:SCR_005393 | |
| Software, algorithm | Kinetics of CA1 pyramidal neuron | https://senselab.med.yale.edu/ModelDB/ShowModel?model=2796#tabs-2 | RRID:SCR_005393 model = 2796#tabs-2 | |
| Software, algorithm | reconstructed CA1 pyramidal neurons | https://senselab.med.yale.edu/ModelDB/ShowModel?model=7509#tabs-1 | RRID:SCR_005393 model = 7509#tabs-1 | |
| Other | Multiclamp 700B | Molecular Devices | RRID:SCR_018455 | |
| Other | Olympus FluoView1000 | Olympus | RRID:SCR_014215 | |
| Other | Femto3D RC | Femtonics | Femto3D RC | |
| Other | BioRad Radiance 2100 | BioRad | Radiance 2100 | |
| Other | Leica VT1200S vibratome | Leica Biosystems | RRID:SCR_018453 |
