(A) Kinetics of infection of L1-130 WT, L1-130 fcpA- vaccine, and L1-130 heat-killed vaccine in blood, kidney, liver, and brain of hamsters after inoculation with 107 bacteria. All animals infected …
Raw data for dissemination experiments in hamster and mouse.
Animals were vaccinated with a dose of 107 leptospires (hamsters) or a range of doses from 107 to 101 leptospires (mice) by subcutaneous (SC) route. Animals were bled the day before immunization …
Raw data for qPCR experiments in hamster and mouse.
Individual sera of hamsters and mice were obtained after 20 days post-vaccination by a subcutaneous (SC) dose of 107 leptospires (hamsters) or a range of doses from 107 to 101 leptospires (mice) of …
Raw data for microscopic agglutination test (MAT) and passive transfer experiments in hamster and mouse, and ELISA data in mouse.
Using statistical modeling we calculated the t-statistics value for each individual antigen used in the proteome array (660 for hamster and 330 for mice) based on three groups: the contrast between …
Raw data of proteome array experiments in hamster and mouse.
Using PSORB information and Genoscope database, we classified the 154 protein targets identified in this study by their putative localization in the cell (A) and their clusters of orthologous group (…
Analysis showing an association between the different doses of the attenuated L1-130 fcpA- attenuated-vaccine in mice and the mean signal response intensity against all different proteins.
Proteins were selected based on the groups depicted on Figure 4 and Supplementary file 4: present in all three groups of analysis (red), present in both hamster and mice immunized with 107 …
Raw data of amino acid identity of 41 selected protein targets among different Leptospira species.
Proteins were selected based on the groups depicted on Figure 4 and Supplementary file 4: present in all three groups of analysis (red), present in both hamster and mice immunized with 107 …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Leptospira interrogans) | Fiocruz L1-130 fcpA- mutant | Wunder et al., 2016a doi:10.1111/mmi.13403 | L. interrogans serovar Copenhageni strain Fiocruz L1-130 fcpA- | Fiocruz L1-130 fcpA- mutant |
Antibody | Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson Immuno Research | Cat# 115-035-166 RRID:AB_2338511 | ELISA (1:50,000) WB (1:100,000) |
Antibody | Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson Immuno Research | Cat# 107-035-142 RRID:AB_2337454 | WB (1:100,000) |
Antibody | Hamster α-fcpA- sera (Hamster polyclonal) | This paper | See Material and methods WB (1:100) | |
Antibody | Hamster control sera (Hamster polyclonal) | This paper | See Material and methods WB (1:100) | |
Chemical compound, drug | SureBlue Reserve TMB 1-Component Microwell Peroxidase Substrate | SeraCare | Cat. #: 5120–0081 | |
Chemical compound, drug | Sulfuric Acid (H2SO4) | Sigma-Aldrich | Cat. #: 258105 | |
Chemical compound, drug | Proteinase K | Thermo Fisher | Cat. #: EO0491 | |
Chemical compound, drug | Platinum Quantitative PCR Supermix-UDG | Thermo Fisher | Cat. #: 11730017 | |
Software, algorithm | GraphPad Prism software | GraphPad Software | RRID:SCR_015807 | Version 8.0.0 |
Software, algorithm | RStudio software | RStudio | RRID:SCR_000432 | Version 1.0.153 |
Leptospira strains used in this study for challenge after vaccination with L1-130 fcpA- mutant.
Efficacy of the immunization with a dose of 107 leptospires of the attenuated L1-130 fcpA- mutant in hamsters followed by challenge with 108 leptospires with homologous or heterologous strains by conjunctival route.
Efficacy of the immunization with different doses of the attenuated L1-130 fcpA- mutant in mice followed by challenge with 108 leptospires of heterologous strain by intraperitoneal route.
Complete list of 154 protein targets identified by the proteome array as correlates of immunity for the attenuated-vaccine model.