(A) Left, cartoon of viral injection site in the ventral striatum. Right, representative confocal images of Cre-dependent PSAM4-GlyR expression (eGFP) in Cre-expressing D1-MSNs (tdTomato). (B) …
PSAM4-GlyR enhances c-fos expression in D1-MSNs in-vivo (source data).
(A) Representative traces of whole-cell voltage-clamp recordings (Vhold −88 mV) demonstrate no effect of uPSEM792 (10 or 50 nM) in control neurons. In PSAM4-GlyR+ neurons, 10 or 50 nM uPSEM792 …
of PSAM4-GlyR depolarizes D1-MSNs (source data).
(A) In PSAM4-GlyR+ neurons, activation of PSAM4-GlyR with uPSEM792 (10 and 50 nM) significantly depolarized the membrane potential (Vm) compared to control (non-PSAM4-GlyR) neurons, measured in …
PSAM4-GlyR does not inhibit firing of D1-MSNs (source data).
(A) Representative traces of cell-attached recordings from a control (gray) or PSAM4-GlyR+ neuron in standard aCSF (4.5 mM [K+]). Action currents were evoked following uPSEM792 by applying a high …
PSAM4-GlyR induces D1-MSN firing in cell-attached recordings (source data).
(A) Current was recorded in response to voltage steps (1 s, −118 to −28 mV, 10 mV) from Vhold −88 mV in aCSF and after application of uPSEM792 (50 or 100 nM). uPSEM792-induced current was isolated …
Current-voltage relationship of PSAM4-GlyR current (source data).
(A) Representative traces of GABA-A receptor-mediated synaptic currents in a PSAM4-GlyR+ neuron in aCSF, evoked during voltage steps from Vhold −88 mV (voltage steps: 1 s, −118 to −28 mV, 10 mV; …
Reduced GABAergic synaptic inhibition (source data).