1. Cell Biology
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Cardiolipin targets a dynamin related protein to the nuclear membrane

  1. Usha Pallabi Kar
  2. Himani Dey
  3. Abdur Rahaman  Is a corresponding author
  1. National Institute of Science Education and Research-HBNI, India
Research Article
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Cite this article as: eLife 2021;10:e64416 doi: 10.7554/eLife.64416

Abstract

Dynamins are targeted to specific cellular membranes that they remodel via membrane fusion or fission. The molecular basis of conferring specificity to dynamins for their target membrane selection is not known. Here, we report a mechanism of nuclear membrane recruitment of Drp6, a dynamin member in Tetrahymena thermophila. Recruitment of Drp6 depends on a domain that binds to cardiolipin-rich bilayers. Consistent with this, nuclear localization of Drp6 was inhibited either by depleting cellular cardiolipin (CL) or by substituting a single amino acid residue that abolished Drp6 interactions with CL. Inhibition of CL synthesis, or perturbation in Drp6 recruitment to nuclear membrane, caused defects in the formation of new macronuclei post-conjugation. Taken together, our results elucidate a molecular basis of target membrane selection by a nuclear dynamin, and establish the importance of a defined membrane-binding domain and its target lipid in facilitating nuclear expansion.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files

Article and author information

Author details

  1. Usha Pallabi Kar

    School of Biological Sciences, National Institute of Science Education and Research-HBNI, Bhubaneswar, India
    Competing interests
    The authors declare that no competing interests exist.
  2. Himani Dey

    School of Biological Sciences, National Institute of Science Education and Research-HBNI, Bhubaneswar, India
    Competing interests
    The authors declare that no competing interests exist.
  3. Abdur Rahaman

    School of Biological Sciences, National Institute of Science Education and Research-HBNI, Bhubaneswar, India
    For correspondence
    arahaman@niser.ac.in
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8440-9633

Funding

Department of Biotechnology, Ministry of Science and Technology, India (BT/PR14643/BRB/10/862/2010)

  • Abdur Rahaman

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Vivek Malhotra, The Barcelona Institute of Science and Technology, Spain

Publication history

  1. Received: October 29, 2020
  2. Accepted: March 3, 2021
  3. Accepted Manuscript published: March 4, 2021 (version 1)
  4. Version of Record published: March 9, 2021 (version 2)

Copyright

© 2021, Kar et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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