Dynamins are targeted to specific cellular membranes that they remodel via membrane fusion or fission. The molecular basis of conferring specificity to dynamins for their target membrane selection is not known. Here, we report a mechanism of nuclear membrane recruitment of Drp6, a dynamin member in Tetrahymena thermophila. Recruitment of Drp6 depends on a domain that binds to cardiolipin-rich bilayers. Consistent with this, nuclear localization of Drp6 was inhibited either by depleting cellular cardiolipin (CL) or by substituting a single amino acid residue that abolished Drp6 interactions with CL. Inhibition of CL synthesis, or perturbation in Drp6 recruitment to nuclear membrane, caused defects in the formation of new macronuclei post-conjugation. Taken together, our results elucidate a molecular basis of target membrane selection by a nuclear dynamin, and establish the importance of a defined membrane-binding domain and its target lipid in facilitating nuclear expansion.
All data generated or analysed during this study are included in the manuscript and supporting files
- Abdur Rahaman
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Vivek Malhotra, The Barcelona Institute of Science and Technology, Spain
© 2021, Kar et al.
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