(A) Magnitude of itch, (B) AUC and (C) Duration of sensation. Data are plotted in the same format as Figure 5H–J for the 25 subjects that, in response to each single pruritogen, reported a greater than zero peak magnitude of both itch and either type of nociceptive sensation (whichever elicited the greater area under the curve [AUC]). This increased the sample size from n = 17 in Figure 5I, J to n = 25 by including the data for eight additional subjects that always felt at least one type of nociceptive sensation but not always both. A 5 ‘stimulus’ × 2 ‘sensory quality’ repeated measures ANOVA (RMANOVA) was performed for peak magnitude, AUC, and duration. For peak magnitude, only ‘stimulus’ had a significant effect (F(4,96)=2.85, p<0.03) with BAM8-22 producing a higher peak magnitude of sensation than ALA (p<0.034, Bonferroni test). The interaction between ‘stimulus’ and ‘sensory quality’ was not significant (F(4,96)=1.89, p=0.12). For AUC, only ‘sensory quality’ was significant (F(1,24.0) = 5.25, p<0.032), with the mean AUC for itch being significantly larger than that for nociceptive sensation (p<0.032, Bonferroni test). The interaction between ‘stimulus’ and ‘sensory quality’ was not significant (F(1.7,42.2) = 0.91, p=0.40). For duration, ‘stimulus’ and ‘sensory quality’ were each significant (F(2.75, 66.0)=4.25, p=0.010 and F(1,24.0) = 13.03, p=0.001, respectively), but their interaction was not (F(2.78, 66,8)=2.1, p=0.11). HIS-induced sensations lasted significantly longer than those induced by ALA or by BAM8-22 + ALA (p<0.01 and p<0.05, respectively). Furthermore, the duration of itch was significantly greater than for the nociceptive sensation (p=0.001, Bonferroni test).