Persistent effects of pair bonding in lung cancer cell growth in monogamous Peromyscus californicus
- Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, United States
- Peromyscus Genetic Stock Center, University of South Carolina, United States
- Department of Physiology, Pharmacology, and Neuroscience, School of Medicine and Department of Laboratory Animal Resources, University of South Carolina, United States
- Department of Pathology, Microbiology and Immunology, School of Medicine, University of South Carolina, United States
Figures
Figure 1 with 1 supplement
Effects of pair bonding in the pro-oncogenic activity of sera.
(a) Representative microphotographs of tumor spheroids developed by A549 cells 3 days after cell seeding. Cells formed spheroids in the presence of sera from bonded (B), bond-disrupted animals (BD) …
Figure 1—figure supplement 1
Microsphere morphology of a panel of human lung cancer cells cultured in P. californicus sera.
Figure 2
Growth of A549 human lung cancers in P. californicus (IS stock) and bonding experience.
Vasectomized males were used in all studies. (a) Volume of measurable tumors at day 15 following cancer cell inoculation in the bonded (n = 9), bond-disrupted (n = 8), and virgin (n = 6) groups; out …
Figure 3 with 3 supplements
RNAseq analysis of A549 cells cultured in the presence of sera from bonded (B), bond-disrupted (BD), or virgin (V) P. californicus.
(a) Bar graphs showing number of differentially expressed genes in each pairwise comparison group. (b ) Volcano plots showing differentially expressed genes between the B vs BD, and B vs V groups. (c…
Figure 3—figure supplement 1
CSC markers in A549 cells cultured in vitro and tumors.
Figure 3—figure supplement 2
Unsupervised hierarchical clustering based on log2 transformed values on all RNAseq data.
Figure 3—figure supplement 3
RNAseq analysis summary of A549 cells.
Figure 4 with 1 supplement
Tumor spheroids with sera from P. polionotus and P. maniculatus.
Morphology of tumor spheroids formed after 2, 3, and 7 days in culture with sera isolated from monogamous P. polionotus and polygamous P. maniculatus. In 6 of 12 P. polionotus but none of 12 P. …
Figure 4—figure supplement 1
Microsphere morphology of A549 human lung cancer cells cultured in P. maniculatus or P. polionotus sera.
Author response image 1
Expression signature of human A549 lung cancer cells cultured in sera from deer mice differing in bonding history.
Author response image 2
Bonding signature and patients’ prognosis.
a. Hierarchical clustering of lung cancer patients from TCGA based on the 13 genes signature and survival. b. Survival probability of the two groups of TCGA lung cancer patients that emerged by …
Tables
Table 1
Differentially expressed genes between the B, BD, and V groups.
Chromosomal location, fold change (log2), and adjusted p-value are indicated. Genes that are common in the B vs BD and V vs B comparisons are underlined.
| Symbol | Chr | log2 fold change | Adj.pval |
|---|---|---|---|
| BD vs B | |||
| FGG | 4q32.1 | 1.49 | 8.82e-04 |
| FGA | 4q31.3 | 1.37 | 8.82e-04 |
| FGB | 4q31.3 | 1.24 | 2.78e-03 |
| HES1 | 3q29 | 1.16 | 3.36e-06 |
| NR4A1 | 12q13.13 | 1.13 | 1.42e-09 |
| SOCS3 | 17q25.3 | 1.09 | 4.39e-10 |
| ZFP36 | 19q13.2 | 1.02 | 1.77e-03 |
| V vs B | |||
| CTGF | 6q23.2 | 1.26 | 5.68e-02 |
| HES1 | 3q29 | 1.18 | 2.26e-06 |
| ZFP36 | 19q13.2 | 1.16 | 9.80e-05 |
| NR4A1 | 12q13.13 | 1.12 | 3.41e-09 |
| FGG | 4q32.1 | 1.08 | 5.39e-02 |
| JUN | 1p32.1 | 1.04 | 3.36e-02 |
| SOCS3 | 17q25.3 | 1.04 | 3.54e-09 |
Table 2
Biological processes associated with the differentially expressed genes in B vs BD and V vs B groups (He et al., 2003).
The adjusted p-values are indicated.
| Group comparison | Adj.pval | Biological process |
|---|---|---|
| BD vs B | 3.1e-06 | Positive regulation of substrate adhesion-dependent cell spreading |
| V vs B | 4.9e-04 | Blood vessel development |
| 5.3e-04 | Positive regulation of intracellular signal transduction | |
| 5.3e-04 | Anatomical structure morphogenesis | |
| 5.3e-04 | Regulation of cellular protein metabolic process | |
| 5.3e-04 | Negative regulation of apoptotic process | |
| 5.3e-04 | Positive regulation of cell differentiation | |
| 5.3e-04 | Regulation of epithelial cell proliferation |
