Developmental emergence of two-stage nonlinear synaptic integration in cerebellar interneurons

  1. Celia Biane
  2. Florian Rückerl
  3. Therese Abrahamsson
  4. Cécile Saint-Cloment
  5. Jean Mariani
  6. Ryuichi Shigemoto
  7. David A DiGregorio
  8. Rachel M Sherrard
  9. Laurence Cathala  Is a corresponding author
  1. Sorbonne Université et CNRS UMR 8256, Adaptation Biologique et Vieillissement, France
  2. Institut Pasteur, Université de Paris, CNRS UMR 3571, Unit of Synapse and Circuit Dynamics, France
  3. Institute of Science and Technology Austria, Austria
  4. Paris Brain Institute, CNRS UMR 7225 - Inserm U1127 – Sorbonne Université Groupe Hospitalier Pitié Salpêtrière, France
8 figures, 1 table and 1 additional file

Figures

Developmental maturation of the AMPA receptor (AMPAR)-mediated miniature excitatory postsynaptic current (mEPSC) in stellate cell (SC).

(A) Left panels show superimposed single mEPSCs (gray) and the corresponding average (bold) recorded at −70 mV obtained from a representative immature SCs (P17, blue trace) and adult SCs (P44, green …

Figure 1—source data 1

Developmental maturation of the AMPAR-mediated mEPSC in SC.

https://cdn.elifesciences.org/articles/65954/elife-65954-fig1-data1-v2.xlsx
Numerical simulations of stellate cell (SC) dendrites indicate significant cable filtering and large local depolarizations in immature SC.

(A) Maximal intensity projection of one-photon confocal images of an immature SC labeled with Alexa 488. Examples of intensity profiles (yellow line) of three dendritic locations from proximal to …

Figure 2—source data 1

Numerical simulations of SC dendrites indicate significant cable filtering and large local depolarizations in immature SC.

https://cdn.elifesciences.org/articles/65954/elife-65954-fig2-data1-v2.xlsx
Quantal excitatory postsynaptic current (EPSC) properties and postsynaptic density (PSD) areas for synapses contacting the soma and dendrites of immature stellate cell (SC).

(A) Diagram of a parasagittal cerebellar slice showing parallel fibers (PFs) projecting perpendicular (blue dots) to the dendritic plane of SCs (in red), allowing precise positioning of the stimulus …

Figure 3—source data 1

qEPSC properties and PSD areas for synapses contacting the soma and dendrites of immature SC.

https://cdn.elifesciences.org/articles/65954/elife-65954-fig3-data1-v2.xlsx
Developmental changes in somatic quantal excitatory postsynaptic current (qEPSC) properties and somatic postsynaptic density (PSD) size.

(A) The left panel shows a diagram of a parasagittal cerebellar slice showing parallel fibers (PFs) projecting perpendicular (blue dots) to the dendritic plane of a stellate cell (SC) (in red), …

Figure 4—source data 1

Developmental changes in somatic qEPSC properties and somatic PSD size.

https://cdn.elifesciences.org/articles/65954/elife-65954-fig4-data1-v2.xlsx
PSD95 puncta distribution on immature and adult stellate cell (SC).

(A) Maximum intensity projection of merged images showing a P14 (top) and P42 (bottom) SC labeled with Alexa 594 (magenta) and Venus-tagged PSD95 puncta (green). (B) Example of single optical …

PSD95 puncta distribution and morphological analysis of immature and adult stellate cell (SC).

(A) Cumulative plot showing Venus-tagged PSD95 puncta distributions for nine immature (blue) and eight adult (green) SCs. The bold trace is the population average (shaded region indicates SEM), …

Figure 6—source data 1

PSD95 puncta distribution and morphological analysis of immature and adult SC.

https://cdn.elifesciences.org/articles/65954/elife-65954-fig6-data1-v2.xlsx
The developmental change in synaptic distribution recapitulates the developmental change in miniature excitatory postsynaptic current (mEPSC) properties.

(A) Numerical simulations of somatic and dendritic quantal excitatory postsynaptic currents (qEPSCs) for synapses placed on the soma (red dot) and at 10 μm intervals (blue dots) along seven of the …

Figure 7—source data 1

The developmental change in synaptic distribution recapitulates the developmental change in mEPSC properties.

https://cdn.elifesciences.org/articles/65954/elife-65954-fig7-data1-v2.xlsx
Location dependence of short-term plasticity and sublinear behavior in immature stellate cell (SC).

(A) Single-trial excitatory postsynaptic current responses (eEPSCs, gray) and their corresponding average (bold) evoked by a pair of electrical stimuli (at 50 Hz).The stimulation electrode was …

Figure 8—source data 1

Location dependence of short-term plasticity and sublinear behavior in immature SC.

https://cdn.elifesciences.org/articles/65954/elife-65954-fig8-data1-v2.xlsx

Tables

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Strain, strain background (Mus musculus – male and female)CB6F1Mouse Genome InformaticsRRID:MGI:5649749
Strain, strain background (Mus musculus – male and female)PSD-95-CreNABLED miceHaining Zhong, Vollum InstituteDOI: https://doi.org/10.1523/JNEUROSCI.3888-14.2014
Strain, strain background (Mus musculus – male and female)B6.129-Nos1tm1(cre)Mgmj/JMouse Genome Informaticshttps://www.jax.org/strain/017526
Chemical compound, drugGabazine (SR-95531)AbcamCat#:ab120042
Chemical compound, drugQX-314AbcamCat#:ab120118
Chemical compound, drug7-Chlorokynurenic acidAbcamCat#:ab120255
Chemical compound, drugAlexa Fluor 488ThermoFisher ScientificCat#:A10436
Chemical compound, drugAlexa Fluor 594ThermoFisher ScientificCat#:A10438
Chemical compound, drugD-AP5AbcamCat#:ab120003
Chemical compound, drugGamma DGGAbcamCat#:ab120307
Chemical compound, drugTTXAbcamCat#:Ab120055
Chemical compound, drugStrychnineSigmaCat#:S0532
Software, algorithmReconstructJC FialaDOI: 10.1111 /j.13652818.2005.01466 .x
Software, algorithmIgor ProWavemetricsRRID:SCR_000325
Software, algorithmNeuromaticRothman and Silver, 2018; DOI:10.3389RRID:SCR_004186
Software, algorithmGraphPad Prism 6GraphPad SoftwareRRID:SCR_002798
Software, algorithmImageJNational Institutes of HealthRRID:SCR_003070
Software, algorithmImarisBitplane Imaris SoftwareRRID:SCR_007370
Software, algorithmZeiss AtlasZeisshttps://www.zeiss.com/microscopy/int/products/microscope-software/atlas.html

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